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Literature summary extracted from

  • Iyer, P.; Ferry, J.G.
    Acetate kinase from Methanosarcina thermophila, a key enzyme for methanogenesis (2005), Methods Biotechnol., 17, 239-246.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.7.2.1 synthesis protocol for overproduction of enzyme Methanosarcina thermophila

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.1 E97D reduction of both Km and kcat-value Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 additional information
-
 additional information assay methods for both directions of reaction Methanosarcina thermophila
2.7.2.1 2.8
-
 acetate mutant E97D, pH 7.5, 37°C Methanosarcina thermophila
2.7.2.1 22
-
 acetate wild-type, pH 7.5, 37°C Methanosarcina thermophila
2.7.2.1 24
-
 propionate wild-type, pH 7.5, 37°C Methanosarcina thermophila

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Methanosarcina thermophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 acetyl phosphate + hydroxylamine
-
 Methanosarcina thermophila acetyl hydroxamate + phosphate
-
 r
2.7.2.1 ATP + acetate
-
 Methanosarcina thermophila ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + butyrate 2% of reactivity with acetate Methanosarcina thermophila ADP + butyryl phosphate
-
 r
2.7.2.1 ATP + ethanol 1% of reactivity with acetate Methanosarcina thermophila ADP + ethyl phosphate
-
 r
2.7.2.1 ATP + formate 2% of reactivity with acetate Methanosarcina thermophila ADP + formyl phosphate
-
 r
2.7.2.1 ATP + glycerol 1% of reactivity with acetate Methanosarcina thermophila ADP + glycerol phosphate
-
 r
2.7.2.1 ATP + glycine 1% of reactivity with acetate Methanosarcina thermophila ADP + glycyl phosphate
-
 r
2.7.2.1 ATP + glycolic acid 4% of reactivity with acetate Methanosarcina thermophila ADP + ?
-
 r
2.7.2.1 ATP + propionate
-
 Methanosarcina thermophila ADP + propionyl phosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.1 190
-
 acetate mutant E97D, pH 7.5, 37°C Methanosarcina thermophila
2.7.2.1 1042
-
 acetate wild-type, pH 7.5, 37°C Methanosarcina thermophila