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Literature summary extracted from

  • Liu, N.; Zhang, T.; Wang, Y.J.; Huang, Y.P.; Ou, J.H.; Shen, P.
    A heat inducible tyrosinase with distinct properties from Bacillus thuringiensis (2004), Lett. Appl. Microbiol., 39, 407-412.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.18.1 EDTA 200-400 mM, activates Bacillus thuringiensis
1.14.18.1 SDS activates Bacillus thuringiensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 alpha,alpha'-dipyridyl 5 mM, 44% inhibition Bacillus thuringiensis
1.14.18.1 diethyldithiocarbamate 0.001 mM, 10% inhibition Bacillus thuringiensis
1.14.18.1 EDTA below 2 mM, 66% inhibition at 0.5 mM, 28% inhibition at 1 mM, no inhibition at 2 mM Bacillus thuringiensis
1.14.18.1 p-phenanthroline 5 M, complete inhibition Bacillus thuringiensis
1.14.18.1 Phenylthiourea 0.001 mM, 26% inhibition Bacillus thuringiensis
1.14.18.1 Sodium fluoride 5 mM, complete inhibition Bacillus thuringiensis
1.14.18.1 Triton X-100 0.5% 87% inhibition Bacillus thuringiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.563
-
 L-Tyr pH 8.5, 42°C Bacillus thuringiensis
1.14.18.1 0.768
-
 L-Dopa pH 8.5, 42°C Bacillus thuringiensis
1.14.18.1 0.892
-
 dopamine pH 8.5, 42°C Bacillus thuringiensis
1.14.18.1 34.05
-
 catechol pH 8.5, 42°C Bacillus thuringiensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 Cu2+ 10 mM, increases activity. Copper-containing enzyme Bacillus thuringiensis
1.14.18.1 Mg2+ 10 mM, increases activity Bacillus thuringiensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 14000
-
 x * 14000, SDS-PAGE Bacillus thuringiensis

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Bacillus thuringiensis
-
 subsp. Kurstaki
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1
-
 Bacillus thuringiensis

Storage Stability

EC Number Storage Stability Organism
1.14.18.1 4°C, 40% loss of activity after 30 days Bacillus thuringiensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 3,4-dihydroxymandelic acid + O2 9% of the activity with L-dopa Bacillus thuringiensis ?
-
 ?
1.14.18.1 3,4-dihydroxyphenylacetic acid + O2 12% of the activity with L-dopa Bacillus thuringiensis ?
-
 ?
1.14.18.1 4-methyl catechol + O2 5% of the activity with L-dopa Bacillus thuringiensis 4-methyl-o-benzoquinone + H2O
-
 ?
1.14.18.1 catechol + 1/2 O2 24% of the activity with L-dopa Bacillus thuringiensis 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 dopamine + 1/2 O2 96% of the activity with L-dopa Bacillus thuringiensis dopamine quinone + H2O
-
 ?
1.14.18.1 L-dopa + 1/2 O2
-
 Bacillus thuringiensis L-dopaquinone + H2O
-
 ?
1.14.18.1 L-Tyr + O2 + AH2 10% of the activity with L-dopa Bacillus thuringiensis L-dopa + H2O + A
-
 ?
1.14.18.1 resorcinol + O2 3% of the activity with L-dopa Bacillus thuringiensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 ? x * 14000, SDS-PAGE Bacillus thuringiensis

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 tyrosinase
-
 Bacillus thuringiensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 75
-
-
 Bacillus thuringiensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.18.1 55 85 55°C: about 75% of maximal activity, 85°C: about 80% of maximal activity Bacillus thuringiensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 85
-
 10 min, 25% loss of activity Bacillus thuringiensis
1.14.18.1 100
-
 10 min, 30% loss of activity. Complete loss of activity after 15 min Bacillus thuringiensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 9
-
-
 Bacillus thuringiensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 8 9.5 pH 8.0: about 30% of maximal activity, pH 9.0: maximal activity, pH 9.5: no activity Bacillus thuringiensis