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Literature summary extracted from

  • Kurian, J.R.; Bajad, S.U.; Miller, J.L.; Chin, N.A.; Trepanier, L.A.
    NADH cytochrome b5 reductase and cytochrome b5 catalyze the microsomal reduction of xenobiotic hydroxylamines and amidoximes in humans (2004), J. Pharmacol. Exp. Ther., 311, 1171-1178.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.2 expressed in Escherichia coli as His-tag fusion protein Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.36
-
 sulfamethoxazole hydroxylamine pH 7.4 Homo sapiens
1.6.2.2 0.36
-
 dapsone hydroxylamine pH 7.4 Homo sapiens
1.6.2.2 0.63
-
 benzamidoxime pH 7.4 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 36000
-
 SDS-PAGE, 32000 Da protein + 4000 Da His-tag Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH Homo sapiens involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 benzamidoxime + NADH Homo sapiens in the presence of cytochrome b5 ?
-
 ?
1.6.2.2 dapsone hydroxylamine + NADH Homo sapiens in the presence of cytochrome b5 ?
-
 ?
1.6.2.2 sulfamethoxazole hydroxylamine + NADH Homo sapiens in the presence of cytochrome b5 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 recombinant protein Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 liver
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
1.6.2.2 4°C, phosphate buffered saline, pH 7.4, protein concentration 2-3 mg/ml, stable for 3 weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH
-
 Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 benzamidoxime + NADH in the presence of cytochrome b5 Homo sapiens ?
-
 ?
1.6.2.2 dapsone hydroxylamine + NADH in the presence of cytochrome b5 Homo sapiens ?
-
 ?
1.6.2.2 sulfamethoxazole hydroxylamine + NADH in the presence of cytochrome b5 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.2.2 diaphorase I
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 FAD
-
 Homo sapiens
1.6.2.2 NADH
-
 Homo sapiens