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Literature summary extracted from

  • Kim, W.; Chae, H.; Park, C.; Lee, K.
    Stability and activity of crosslinking enzyme crystals of cyclodextrin glucanotransferase isolated from Bacillus macerans (2003), J. Mol. Catal. B, 26, 287-292.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.19 hanging drop vapor diffusion technique. Cross-linked enzyme crystals can be useful biocatalysts because they are stable at elevated temperature, in organic solvents, and in the presence of enzyme inactivation surfactant. They also maintain their activity against protein-digesting enzyme Paenibacillus macerans

General Stability

EC Number General Stability Organism
2.4.1.19 cross-linked enzyme crystals are slightly more resistant to 2-butanol and acetonitrile than DMSO. In 15% solution of 2-butanol, over 100% of the activity is retained in CGTase-cross-linking enzyme crystals while 28% of the activity remains in the case of soluble CGTase Paenibacillus macerans
2.4.1.19 SDS, 1%, only 7% of the activity of soluble enzyme activity remains, cross-linked enzyme crystals exhibit strong activity Paenibacillus macerans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.19 HgCl2 5 mM, 68% inhibition of cross-linked enzyme crystals, 40% of soluble enzyme Paenibacillus macerans
2.4.1.19 MgCl2 5 mM, 15% inhibition of cross-linked enzyme crystals, 22% of soluble enzyme Paenibacillus macerans
2.4.1.19 ZnCl2 5 mM, 30% inhibition of cross-linked enzyme crystals, 80% of soluble enzyme Paenibacillus macerans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.19 CaCl2 5 mM, 20% activation of cross-linked enzyme crystals, 2% activation of soluble enzyme Paenibacillus macerans
2.4.1.19 FeCl2 5 mM, 110% activation of cross-linked enzyme crystals, inhibition of soluble enzyme Paenibacillus macerans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.4.1.19 2-butanol in 15% solution of 2-butanol, over 100% of the activity is retained in CGTase-cross-linking enzyme crystals while 28% of the activity remains in the case of soluble CGTase Paenibacillus macerans
2.4.1.19 acetonitrile in 15% solution of 2-butanol, over 100% of the activity is retained in CGTase-cross-linking enzyme crystals while 60% of the activity remains in the case of soluble CGTase Paenibacillus macerans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Paenibacillus macerans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans cyclodextrin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 60
-
 soluble enzyme Paenibacillus macerans
2.4.1.19 60 70 cross-linked enzyme crystals Paenibacillus macerans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.19 40 80 40°C: about 60% of maximal activity, 80°C: about 45% of maximal activity,cross-linked enzyme crystals Paenibacillus macerans
2.4.1.19 40 75 40°C: about 40% of maximal activity, 75°C: about 15% of maximal activity, soluble enzyme Paenibacillus macerans