Literature summary extracted from
Kim, W.; Chae, H.; Park, C.; Lee, K.
Stability and activity of crosslinking enzyme crystals of cyclodextrin glucanotransferase isolated from Bacillus macerans (2003), J. Mol. Catal. B, 26, 287-292.
No PubMed abstract available
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.4.1.19 |
hanging drop vapor diffusion technique. Cross-linked enzyme crystals can be useful biocatalysts because they are stable at elevated temperature, in organic solvents, and in the presence of enzyme inactivation surfactant. They also maintain their activity against protein-digesting enzyme |
Paenibacillus macerans |
General Stability
EC Number |
General Stability |
Organism |
---|
2.4.1.19 |
cross-linked enzyme crystals are slightly more resistant to 2-butanol and acetonitrile than DMSO. In 15% solution of 2-butanol, over 100% of the activity is retained in CGTase-cross-linking enzyme crystals while 28% of the activity remains in the case of soluble CGTase |
Paenibacillus macerans |
2.4.1.19 |
SDS, 1%, only 7% of the activity of soluble enzyme activity remains, cross-linked enzyme crystals exhibit strong activity |
Paenibacillus macerans |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.4.1.19 |
HgCl2 |
5 mM, 68% inhibition of cross-linked enzyme crystals, 40% of soluble enzyme |
Paenibacillus macerans |
|
2.4.1.19 |
MgCl2 |
5 mM, 15% inhibition of cross-linked enzyme crystals, 22% of soluble enzyme |
Paenibacillus macerans |
|
2.4.1.19 |
ZnCl2 |
5 mM, 30% inhibition of cross-linked enzyme crystals, 80% of soluble enzyme |
Paenibacillus macerans |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.4.1.19 |
CaCl2 |
5 mM, 20% activation of cross-linked enzyme crystals, 2% activation of soluble enzyme |
Paenibacillus macerans |
|
2.4.1.19 |
FeCl2 |
5 mM, 110% activation of cross-linked enzyme crystals, inhibition of soluble enzyme |
Paenibacillus macerans |
|
Organic Solvent Stability
EC Number |
Organic Solvent |
Comment |
Organism |
---|
2.4.1.19 |
2-butanol |
in 15% solution of 2-butanol, over 100% of the activity is retained in CGTase-cross-linking enzyme crystals while 28% of the activity remains in the case of soluble CGTase |
Paenibacillus macerans |
2.4.1.19 |
acetonitrile |
in 15% solution of 2-butanol, over 100% of the activity is retained in CGTase-cross-linking enzyme crystals while 60% of the activity remains in the case of soluble CGTase |
Paenibacillus macerans |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.1.19 |
Paenibacillus macerans |
- |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.4.1.19 |
soluble starch + glycosyl acceptor |
- |
Paenibacillus macerans |
cyclodextrin |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.4.1.19 |
60 |
- |
soluble enzyme |
Paenibacillus macerans |
2.4.1.19 |
60 |
70 |
cross-linked enzyme crystals |
Paenibacillus macerans |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
2.4.1.19 |
40 |
80 |
40°C: about 60% of maximal activity, 80°C: about 45% of maximal activity,cross-linked enzyme crystals |
Paenibacillus macerans |
2.4.1.19 |
40 |
75 |
40°C: about 40% of maximal activity, 75°C: about 15% of maximal activity, soluble enzyme |
Paenibacillus macerans |