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Literature summary extracted from

  • Kim, I.J.; Ko, K.C.; Nam, T.S.; Kim, Y.W.; Chung, W.I.; Kim, C.S.
    Expression and activity of citrus phytoene synthase and beta-carotene hydroxylase in Escherichia coli (2003), J. Microbiol., 41, 212-218.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.24 expressed in Escherichia coli Pantoea ananatis
2.5.1.32 gene crtB, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme lacking the chloroplast transit peptide in Escherichia coli strain BL21(DE3) mostly in inclusion bodies Citrus sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.32 additional information recombinant in vivo reconstitution of carotinoid biosynthesis by expression of genes crtE, crtX, crtY, crtI, and crtB encoding enzymes involved in the pathway from Pantoea ananatis and citrus genes crtB and crtZ encoding CitPsy and CitChx, i.e. phytoene synthase and beta-carotene hydroxylase, in Escherichia coli strain BL21(DE3), carotinoid pigment content analysis of recombinant bacteria, overview Citrus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.32 chloroplast enzyme contains a chloroplast transit peptide Citrus sp. 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.32 28000
-
 x * 28000, about, recombinant enzyme, SDS-PAGE Citrus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.32 geranylgeranyl diphosphate + geranylgeranyl diphosphate Citrus sp. enzyme is involved in carotenoid biosynthesis, pathway overview prephytoene diphosphate + diphosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.24 Pantoea ananatis
-
 formerly Erwinia uredovora
-
2.5.1.32 Citrus sp.
-
 gene crtB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 beta-carotene is only partly converted into zeaxanthin by Chx, though a major portion (75-80%) is left Pantoea ananatis zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
2.5.1.32 geranylgeranyl diphosphate + geranylgeranyl diphosphate
-
 Citrus sp. prephytoene diphosphate + diphosphate
-
 ir
2.5.1.32 geranylgeranyl diphosphate + geranylgeranyl diphosphate enzyme is involved in carotenoid biosynthesis, pathway overview Citrus sp. prephytoene diphosphate + diphosphate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.5.1.32 ? x * 28000, about, recombinant enzyme, SDS-PAGE Citrus sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.15.24 beta-carotene hydroxylase
-
 Pantoea ananatis
1.14.15.24 Chx
-
 Pantoea ananatis
1.14.15.24 CHX1
-
 Pantoea ananatis
2.5.1.32 CitPsy
-
 Citrus sp.
2.5.1.32 PSY
-
 Citrus sp.