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Literature summary extracted from

  • Yen, C.L.; Brown Iv, C.H.; Monetti, M.; Farese, R.V., Jr.
    A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters (2005), J. Lipid Res., 46, 2388-2397.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 chromosomal location, expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells and in COS-7 cells Homo sapiens
2.3.1.22 chromosomal location, expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells and in COS-7 cells Homo sapiens
2.3.1.75 chromosomal location, expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells and in COS-7 cells Homo sapiens
2.3.1.75 MFAT, DNA and amino acid sequence determination and analysis, localized on the X chromosome, expression of the N-terminally FLAG-tagged enzyme in Spodoptera frugierda Sf9 cells using the baculovirus transfection system, or in COS-7 cells Homo sapiens
2.3.1.76 chromosomal location, expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells and in COS-7 cells Homo sapiens
2.3.1.76 expression in insect cells or in COS-7 cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 membrane
-
 Homo sapiens 16020
-
2.3.1.22 membrane
-
 Homo sapiens 16020
-
2.3.1.75 membrane
-
 Homo sapiens 16020
-
2.3.1.76 membrane
-
 Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.20 Mg2+
-
 Homo sapiens
2.3.1.22 Mg2+
-
 Homo sapiens
2.3.1.75 Mg2+
-
 Homo sapiens
2.3.1.76 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 1,2-dioleoylglycerol + palmitoyl-CoA Homo sapiens
-
 1,2-dioleoyl-3-palmitoylglycerol + CoA
-
 ?
2.3.1.20 additional information Homo sapiens the multifunctional enzyme plays an important role in lipid metabolism in human skin ?
-
 ?
2.3.1.22 2-monooleoylglycerol + palmitoyl-CoA Homo sapiens
-
 2-oleoyl-3-palmitoylglycerol + CoA
-
 ?
2.3.1.22 additional information Homo sapiens the multifunctional enzyme plays an important role in lipid metabolism in human skin ?
-
 ?
2.3.1.75 additional information Homo sapiens the enzyme plays an important role in lipid metabolism in human skin, overview ?
-
 ?
2.3.1.75 additional information Homo sapiens the multifunctional enzyme plays an important role in lipid metabolism in human skin ?
-
 ?
2.3.1.76 additional information Homo sapiens the multifunctional O-acyltransferase likely plays an important role in lipid metabolism in human skin ?
-
 ?
2.3.1.76 additional information Homo sapiens the multifunctional enzyme plays an important role in lipid metabolism in human skin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Homo sapiens
-
-
-
2.3.1.22 Homo sapiens
-
-
-
2.3.1.75 Homo sapiens
-
-
-
2.3.1.75 Homo sapiens Q6E213 MFAT
-
2.3.1.76 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 adipose tissue
-
 Homo sapiens
-
2.3.1.20 liver very low expression level Homo sapiens
-
2.3.1.20 lung
-
 Homo sapiens
-
2.3.1.20 pancreas very low expression level Homo sapiens
-
2.3.1.20 skin high expression level Homo sapiens
-
2.3.1.20 testis
-
 Homo sapiens
-
2.3.1.22 adipose tissue
-
 Homo sapiens
-
2.3.1.22 liver very low expression level Homo sapiens
-
2.3.1.22 lung
-
 Homo sapiens
-
2.3.1.22 pancreas very low expression level Homo sapiens
-
2.3.1.22 skin high expression level Homo sapiens
-
2.3.1.22 testis
-
 Homo sapiens
-
2.3.1.75 adipose tissue
-
 Homo sapiens
-
2.3.1.75 liver very low expression level Homo sapiens
-
2.3.1.75 lung
-
 Homo sapiens
-
2.3.1.75 lung very low expression level Homo sapiens
-
2.3.1.75 pancreas very low expression level Homo sapiens
-
2.3.1.75 skin predominantly Homo sapiens
-
2.3.1.75 skin high expression level Homo sapiens
-
2.3.1.75 testis
-
 Homo sapiens
-
2.3.1.75 testis very low expression level Homo sapiens
-
2.3.1.76 adipose tissue
-
 Homo sapiens
-
2.3.1.76 liver very low expression level Homo sapiens
-
2.3.1.76 lung
-
 Homo sapiens
-
2.3.1.76 pancreas very low expression level Homo sapiens
-
2.3.1.76 skin highly expressed in Homo sapiens
-
2.3.1.76 skin high expression level Homo sapiens
-
2.3.1.76 testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 1,2-dioleoylglycerol + palmitoyl-CoA
-
 Homo sapiens 1,2-dioleoyl-3-palmitoylglycerol + CoA
-
 ?
2.3.1.20 additional information the multifunctional enzyme plays an important role in lipid metabolism in human skin Homo sapiens ?
-
 ?
2.3.1.20 additional information DGAT1 is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview Homo sapiens ?
-
 ?
2.3.1.22 2-monooleoylglycerol + palmitoyl-CoA
-
 Homo sapiens 2-oleoyl-3-palmitoylglycerol + CoA
-
 ?
2.3.1.22 additional information the multifunctional enzyme plays an important role in lipid metabolism in human skin Homo sapiens ?
-
 ?
2.3.1.22 additional information the multifunctional O-acetyltransferase is catalyzing the reactions of the monoacylglycerol transferase, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview Homo sapiens ?
-
 ?
2.3.1.75 1,2-hexadecandiol + palmitoyl-CoA
-
 Homo sapiens ?
-
 ?
2.3.1.75 1-hexadecanol + palmitoyl-CoA
-
 Homo sapiens CoA + hexadecylpalmitate
-
 ?
2.3.1.75 additional information the enzyme plays an important role in lipid metabolism in human skin, overview Homo sapiens ?
-
 ?
2.3.1.75 additional information the multifunctional enzyme plays an important role in lipid metabolism in human skin Homo sapiens ?
-
 ?
2.3.1.75 additional information substrate specificity, overview, a human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters, overview, the enzyme catalyzes also the reactions of EC 2.3.1.20 and EC 2.3.1.22 Homo sapiens ?
-
 ?
2.3.1.75 additional information the multifunctional O-acetyltransferase is catalyzing the reactions of the wax synthase, of the diacylglycerol transferase, EC 2.3.1.20, of the monoacylglycerol transferase, EC 2.3.1.22, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview Homo sapiens ?
-
 ?
2.3.1.75 palmitoyl-CoA + 1,2-hexadecandiol
-
 Homo sapiens ?
-
 ?
2.3.1.75 palmitoyl-CoA + all-trans-retinol
-
 Homo sapiens ?
-
 ?
2.3.1.75 palmitoyl-CoA + dioleoylglycerol
-
 Homo sapiens ?
-
 ?
2.3.1.75 palmitoyl-CoA + hexadecanol
-
 Homo sapiens CoA + hexadecyl palmitate
-
 ?
2.3.1.75 palmitoyl-CoA + monooleoylglycerol
-
 Homo sapiens ?
-
 ?
2.3.1.76 all-trans retinol + palmitoyl-CoA
-
 Homo sapiens all-trans-retinyl palmitate + CoA
-
 ?
2.3.1.76 additional information the multifunctional O-acyltransferase likely plays an important role in lipid metabolism in human skin Homo sapiens ?
-
 ?
2.3.1.76 additional information the multifunctional enzyme plays an important role in lipid metabolism in human skin Homo sapiens ?
-
 ?
2.3.1.76 additional information the multifunctional O-acetyltransferase is catalyzing the reactions of the acyl-CoA:retinol acyltransferase, of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, and of the wax synthase, EC 2.3.1.75, overview Homo sapiens ?
-
 ?
2.3.1.76 palmitoyl-CoA + retinol
-
 Homo sapiens CoA + retinylpalmitate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 acyl-CoA:diacylglycerol acyltransferase 2
-
 Homo sapiens
2.3.1.20 MFAT
-
 Homo sapiens
2.3.1.20 More the enzyme belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase, MGAT, gene family Homo sapiens
2.3.1.20 multifunctional O-acyltransferase
-
 Homo sapiens
2.3.1.22 acyl-CoA:monoacylglycerol acyltransferase
-
 Homo sapiens
2.3.1.22 MFAT
-
 Homo sapiens
2.3.1.22 MGAT
-
 Homo sapiens
2.3.1.22 More the enzyme belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase, MGAT, gene family Homo sapiens
2.3.1.22 multifunctional O-acyltransferase
-
 Homo sapiens
2.3.1.75 acyl-CoA:fatty acyl alcohol acyltransferase
-
 Homo sapiens
2.3.1.75 MFAT
-
 Homo sapiens
2.3.1.75 More cf. EC 2.3.1.20 and EC 2.3.1.22, the enzyme belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase, MGAT, gene family Homo sapiens
2.3.1.75 More the enzyme belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase, MGAT, gene family Homo sapiens
2.3.1.75 multifunctional O-acyltransferase
-
 Homo sapiens
2.3.1.75 wax synthase
-
 Homo sapiens
2.3.1.76 acyl-CoA:retinol acyltransferase
-
 Homo sapiens
2.3.1.76 ArAT
-
 Homo sapiens
2.3.1.76 MFAT
-
 Homo sapiens
2.3.1.76 More the enzyme belongs to the acyl-CoA:diacylglycerol acyltransferase 2/acyl-CoA:monoacylglycerol acyltransferase, MGAT, gene family Homo sapiens
2.3.1.76 multifunctional O-acyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 25
-
 assay at Homo sapiens
2.3.1.22 25
-
 assay at Homo sapiens
2.3.1.75 25
-
 assay at Homo sapiens
2.3.1.75 37
-
 assay at Homo sapiens
2.3.1.76 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7.4
-
 assay at Homo sapiens
2.3.1.22 7.4
-
 assay at Homo sapiens
2.3.1.75 7.4
-
 assay at Homo sapiens
2.3.1.76 7.4
-
 assay at Homo sapiens