Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Barnes, S.; Burhol, P.G.; Zander, R.; Haggstrom, G.; Settine, R.L.; Hirschowitz, B.I.
    Enzymic sulfation of glycochenodeoxycholic acid by tissue fractions from adult hamsters (1979), J. Lipid Res., 20, 952-959.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.34 chenodeoxycholate competitive, 50% inhibition at about 0.1 mM Mesocricetus auratus
2.8.2.34 glycolithocholate competitive, 50% inhibition at 0.04 mM Mesocricetus auratus
2.8.2.34 ursodeoxycholate competitive, 50% inhibition at about 0.1 mM Mesocricetus auratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.34 additional information
-
 additional information
-
 Mesocricetus auratus
2.8.2.34 0.00023
-
 glycochenodeoxycholate male hamster, pH 7.0, 37°C, physiological substrate concentration Mesocricetus auratus
2.8.2.34 0.00142
-
 glycochenodeoxycholate female hamster, pH 7.0, 37°C, physiological substrate concentration Mesocricetus auratus
2.8.2.34 0.079
-
 glycochenodeoxycholate female hamster, pH 7.0, 37°C, saturating substrate concentration Mesocricetus auratus
2.8.2.34 0.317
-
 glycochenodeoxycholate male hamster, pH 7.0, 37°C, saturating substrate concentration Mesocricetus auratus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.34 soluble
-
 Mesocricetus auratus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.34 Mg2+ required for activity, best at 2.5 mM Mesocricetus auratus
2.8.2.34 additional information activity is independent of ionic strength in the range of 25-125 mM Mesocricetus auratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.34 3'-phosphoadenylyl 5'-phosphosulfate + glycochenodeoxycholate Mesocricetus auratus
-
 adenosine 3',5'-bisphosphate + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.34 Mesocricetus auratus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.34 adrenal gland
-
 Mesocricetus auratus
-
2.8.2.34 intestine proximal Mesocricetus auratus
-
2.8.2.34 liver
-
 Mesocricetus auratus
-
2.8.2.34 additional information no activity in herat, spleen, skeletal muscle, brain, distal small intestine, and large intestine, activity is higher in femal than in male hamsters Mesocricetus auratus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.34 0.000003
-
 adrenal gland supernatant fraction Mesocricetus auratus
2.8.2.34 0.000004
-
 proximal intestine supernatant fraction, female hamster Mesocricetus auratus
2.8.2.34 0.000005
-
 proximal intestine supernatant fraction, male hamster Mesocricetus auratus
2.8.2.34 0.000017
-
 liver supernatant fraction, male hamster Mesocricetus auratus
2.8.2.34 0.000062
-
 liver supernatant fraction, female hamster Mesocricetus auratus

Storage Stability

EC Number Storage Stability Organism
2.8.2.34 -20°C, hepatic and intestinal supernatant fractions, completely stable for at least 6 months Mesocricetus auratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.34 3'-phosphoadenylyl 5'-phosphosulfate + glycochenodeoxycholate
-
 Mesocricetus auratus adenosine 3',5'-bisphosphate + ?
-
 ?
2.8.2.34 3'-phosphoadenylyl 5'-phosphosulfate + glycochenodeoxycholate
-
 Mesocricetus auratus adenosine 3',5'-bisphosphate + ? product identification ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.34 BAST
-
 Mesocricetus auratus
2.8.2.34 bile acid:PAPS:sulfotransferase
-
 Mesocricetus auratus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.34 37
-
 assay at Mesocricetus auratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.34 7
-
-
 Mesocricetus auratus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.34 6 8
-
 Mesocricetus auratus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.34 0.018
-
 glycolithocholate pH 7.0, 37°C Mesocricetus auratus
2.8.2.34 0.071
-
 chenodeoxycholate pH 7.0, 37°C Mesocricetus auratus
2.8.2.34 0.083
-
 ursodeoxycholate pH 7.0, 37°C Mesocricetus auratus