EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.1 | dimethylsulfoxid | up to 25% v/v | Bacillus subtilis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.1 | - |
Bacillus subtilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.1 | A74G/F88V | substrate specificity similar to wild-type | Bacillus subtilis |
1.14.14.1 | A74G/F88V/S189Q | substrate spectrum much broader than for wild-type | Bacillus subtilis |
1.14.14.1 | A74G/S189Q | substrate specificity similar to wild-type | Bacillus subtilis |
EC Number | General Stability | Organism |
---|---|---|
1.14.14.1 | not stable in NaCl above 150 mM | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.1 | 0.0064 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
1.14.14.1 | 0.0065 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
1.14.14.1 | 0.0104 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
1.14.14.1 | 0.0245 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
1.14.14.1 | 0.0319 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
1.14.14.1 | 0.0618 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, wild-type | Bacillus subtilis |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.14.14.1 | DMSO | stable, and activity increases in presence of up to 25% DMSO (v/v) | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.1 | Bacillus subtilis | - |
isozyme CYP102A3, natural fusion protein consisting of a heme domain and a reductase domain | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.1 | beta-ionone + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | capric acid + [reduced NADPH-hemoprotein reductase] + O2 | about 10% of the activity with palmitic acid, wild-type | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | caprylic acid + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | lauric acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | naphthalene + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | octane + [reduced NADPH-hemoprotein reductase] + O2 | substrate only for mutant A74G/F88V/S189Q and for mutant A74G/S189Q | Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | omega-(p-nitrophenyl)decanoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | omega-(p-nitrophenyl)dodecanoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | omega-(p-nitrophenyl)octanoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.1 | palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bacillus subtilis | ? + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.1 | 0.4 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
1.14.14.1 | 1.3 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
1.14.14.1 | 1.3 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis | |
1.14.14.1 | 1.5 | - |
omega-(p-nitrophenyl)decanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
1.14.14.1 | 3.1 | - |
omega-(p-nitrophenyl)dodecanoic acid | pH 8.0, wild-type | Bacillus subtilis | |
1.14.14.1 | 3.5 | - |
omega-(p-nitrophenyl)octanoic acid | pH 8.0, mutant A74G/F88V/S189Q | Bacillus subtilis |