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Literature summary extracted from

  • Wang, T.P.; Quintanar, L.; Severance, S.; Solomon, E.I.; Kosman, D.J.
    Targeted suppression of the ferroxidase and iron trafficking activities of the multicopper oxidase Fet3p from Saccharomyces cerevisiae (2003), J. Biol. Inorg. Chem., 8, 611-620.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.16.3.1 D278A normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type, in crease in Km-value compared to wild-type Saccharomyces cerevisiae
1.16.3.1 E185A normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type, in crease in Km-value compared to wild-type, inactive in support of iron uptake Saccharomyces cerevisiae
1.16.3.1 E185A/Y354A normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type, in crease in Km-value compared to wild-type Saccharomyces cerevisiae
1.16.3.1 E185D normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type Saccharomyces cerevisiae
1.16.3.1 Y354A normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type, in crease in Km-value compared to wild-type Saccharomyces cerevisiae
1.16.3.1 Y354F normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.16.3.1 0.0048
-
 Fe2+ wild-type, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.005
-
 p-phenylenediamine mutant D278A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.0058
-
 Fe2+ mutant Y354F, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.008
-
 p-phenylenediamine mutant Y354A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.0084
-
 p-phenylenediamine wild-type, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.0086
-
 Fe2+ mutant E185D, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.011
-
 Fe2+ mutant D278A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.0143
-
 p-phenylenediamine mutant Y354F, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.017
-
 Fe2+ mutant Y354A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.0228
-
 p-phenylenediamine mutant E185D, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.0333
-
 p-phenylenediamine mutant E185A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.04
-
 Fe2+ mutant E185A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.0705
-
 p-phenylenediamine mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 3
-
 hydroquinone wild-type, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 3.51
-
 Fe2+ mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 4.3
-
 hydroquinone mutant D278A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 4.6
-
 hydroquinone mutant Y354A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 8.2
-
 hydroquinone mutant E185D, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 12.7
-
 hydroquinone mutant Y354F, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 14.1
-
 hydroquinone mutant E185A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 16.9
-
 hydroquinone mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.16.3.1 plasma membrane outer surface Saccharomyces cerevisiae 5886
-

Organism

EC Number Organism UniProt Comment Textmining
1.16.3.1 Saccharomyces cerevisiae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.3.1 hydroquinone + Fe2+ + O2
-
 Saccharomyces cerevisiae ?
-
 ?
1.16.3.1 p-phenylenediamine + Fe2+ + O2
-
 Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.16.3.1 multicopper oxidase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.16.3.1 0.37
-
 p-phenylenediamine mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.39
-
 hydroquinone mutant E185A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.48
-
 Fe2+ mutant E185A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.5
-
 Fe2+ mutant D278A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.52
-
 Fe2+ mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.52
-
 hydroquinone mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.55
-
 Fe2+ mutant E185D, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.56
-
 Fe2+ mutant Y354A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.62
-
 hydroquinone mutant D278A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.62
-
 p-phenylenediamine mutant D278A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.68
-
 Fe2+ mutant Y354F, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.78
-
 Fe2+ wild-type, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.85
-
 hydroquinone wild-type, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.92
-
 p-phenylenediamine wild-type, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 0.98
-
 p-phenylenediamine mutant E185A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 1.18
-
 p-phenylenediamine mutant Y354F, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 1.57
-
 hydroquinone mutant E185D, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 1.7
-
 hydroquinone mutant Y354F, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 1.73
-
 p-phenylenediamine mutant E185D, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 4.8
-
 p-phenylenediamine mutant Y354A, pH 6.0 Saccharomyces cerevisiae
1.16.3.1 7.2
-
 hydroquinone mutant Y354A, pH 6.0 Saccharomyces cerevisiae