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Literature summary extracted from

  • Blondal, T.; Hjorleifsdottir, S.; Aevarsson, A.; Fridjonsson, O.H.; Skirnisdottir, S.; Wheat, J.O.; Hermannsdottir, A.G.; Hreggvidsson, G.O.; Smith, A.V.; Kristjansson, J.K.
    Characterization of a 5'-polynucleotide kinase/3'-phosphatase from bacteriophage RM378 (2005), J. Biol. Chem., 280, 5188-5194.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.78 PEG 6000 5-15%, 3-4fold increase in activity, inhibitory above Rhodothermus phage RM378

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.32 expression in Escherichia coli Rhodothermus phage RM378

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 ADP complete inhibition at 1 mM Rhodothermus phage RM378
2.7.1.78 KCl inhibitory above 10 mM Rhodothermus phage RM378
2.7.1.78 Mn2+ maximum activity at 0.01 mM, inhibitory above 0.1 mM Rhodothermus phage RM378
2.7.1.78 NaCl inhibitory above 10 mM Rhodothermus phage RM378
2.7.1.78 PEG 6000 5-15%, 3-4fold increase in activity, inhibitory above Rhodothermus phage RM378

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.78 0.0013
-
 r(A20) pH 6.0, 65°C Rhodothermus phage RM378
2.7.1.78 0.0015
-
 r(A20) pH 6.0, 65°C Rhodothermus phage RM378
2.7.1.78 0.02
-
 ATP pH 6.0, 65°C Rhodothermus phage RM378
3.1.3.32 0.006
-
 3'-TMP
-
 Rhodothermus phage RM378
3.1.3.32 0.7
-
 cAMP
-
 Rhodothermus phage RM378

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 Mg2+ maximum activity at 0.1 mM Rhodothermus phage RM378
2.7.1.78 Mn2+ maximum activity at 0.01 mM, inhibitory above 0.1 mM Rhodothermus phage RM378
2.7.1.78 additional information dovalent metal ion required Rhodothermus phage RM378
3.1.3.32 Mg2+ activity relatively low on cAMP at pH 6 using Mg2+. When Mg2+ is replaced with Mn2+ in the 3'-phosphohydrolase assay on the cAMP substrate, the activity increases, resulting in an 10fold increase in activity at 1 mM concentration Rhodothermus phage RM378
3.1.3.32 Mn2+ activity relatively low on cAMP at pH 6 using Mg2+. When Mg2+ is replaced with Mn2+ in the 3'-phosphohydrolase assay on the cAMP substrate, the activity increases, resulting in an 10fold increase in activity at 1 mM concentration Rhodothermus phage RM378

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Rhodothermus phage RM378
-
 infecting Rhodothermus marinus, bifunctional enzyme with 5’-kinase domain and 3’-phosphohydrolase domain
-
3.1.3.32 Rhodothermus phage RM378
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.78 2.67
-
 substrate r(A20), pH 6.0, 65°C Rhodothermus phage RM378
2.7.1.78 3.67
-
 substrate d(A20), pH 6.0, 65°C Rhodothermus phage RM378

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA acts both on single- and blunt end double-stranded DNA Rhodothermus phage RM378 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA
-
 Rhodothermus phage RM378 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + d(A20)
-
 Rhodothermus phage RM378 ADP + 5'-phospho-d(A20)
-
 ?
2.7.1.78 ATP + r(A20)
-
 Rhodothermus phage RM378 ADP + 5'-phospho-r(A20)
-
 ?
3.1.3.32 3'-TMP + H2O
-
 Rhodothermus phage RM378 ?
-
 ?
3.1.3.32 cAMP + H2O
-
 Rhodothermus phage RM378 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.32 5'-polynucleotide kinase/3'-phosphatase the polynucleotide kinase has a 5'-kinase domain as well as a 3'-phosphohydrolase domain Rhodothermus phage RM378

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.78 60 70
-
 Rhodothermus phage RM378
3.1.3.32 75
-
-
 Rhodothermus phage RM378

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.32 60 80 60°C: about 50% of maximal activity, 80°C: about 80% of maximal activity Rhodothermus phage RM378

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.78 65
-
 stable up to Rhodothermus phage RM378
2.7.1.78 70
-
 stable for 1 h Rhodothermus phage RM378
3.1.3.32 60 65 2 h, stable up to Rhodothermus phage RM378

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.78 1.9
-
 r(A20) pH 6.0, 65°C Rhodothermus phage RM378
2.7.1.78 2.6
-
 r(A20) pH 6.0, 65°C Rhodothermus phage RM378
3.1.3.32 0.017
-
 cAMP
-
 Rhodothermus phage RM378
3.1.3.32 0.16
-
 3'-TMP
-
 Rhodothermus phage RM378

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 8.5
-
-
 Rhodothermus phage RM378
3.1.3.32 6
-
-
 Rhodothermus phage RM378

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.78 7.5 10 more than 80% of maximum activity Rhodothermus phage RM378
3.1.3.32 5.5 7 pH 5.5: about 50% of maximal activity, pH 7.0: abput 35% of maximal activity Rhodothermus phage RM378