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Literature summary extracted from

  • Zhang, L.; Joshi, A.K.; Hofmann, J.; Schweizer, E.; Smith, S.
    Cloning, expression, and characterization of the human mitochondrial beta-ketoacyl synthase. Complementation of the yeast CEM1 knock-out strain (2005), J. Biol. Chem., 280, 12422-12429.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 DNA and amino acid sequence determination and analysis, phylogenetic tree, expression as GFP-fusion protein containing the mitochondrial targeting sequence in mitochondria of HeLa cells, expression of truncated enzyme forms lacking the first 21 or 59 amino acid residues, respectively, in Escherichia coli strain BL21(DE3) as soluble enzymes, or in Spodoptera frugiperda Sf9 cells via baculovirus infection system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 cerulenin 50% inhibition at 0.2 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.41 0.0018
-
decanoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 0.0019
-
hexanoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 0.0039
-
butanoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 0.0095
-
dodecanoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 0.0109
-
octanoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 0.05
-
acetyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 0.0508
-
myristoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.39 mitochondrion enzyme is part of the malonyl-CoA-dependent type II fatty acid synthase complex in mitochondria, and contains a mitochondrial targeting sequence Homo sapiens 5739
-
2.3.1.41 mitochondrion
-
Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 45800
-
x * 45800, SDS-PAGE, recombinant protein without N-terminal targeting sequence, with His-tag Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.39 malonyl-CoA + [acyl-carrier protein] Homo sapiens absolutely specific for the substrates, type II [acyl-carrier protein] CoA + malonyl-[acyl-carrier protein]
-
?
2.3.1.39 additional information Homo sapiens enzyme is part of the malonyl-CoA-dependent type II fatty acid synthase complex in mitochondria ?
-
?
2.3.1.41 additional information Homo sapiens important role in generation of the lipoic acid precursor octanoyl-acyl-carrier protein ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Homo sapiens
-
-
-
2.3.1.41 Homo sapiens
-
expression in Escherichia coli, without N-terminal targeting sequence
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.41 proteolytic modification mitochondrial targeting sequence of 38 residues Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 recombinant truncated enzyme forms from Sf9 insect cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.41 brain low level Homo sapiens
-
2.3.1.41 heart most abundant Homo sapiens
-
2.3.1.41 kidney
-
Homo sapiens
-
2.3.1.41 liver
-
Homo sapiens
-
2.3.1.41 lung low level Homo sapiens
-
2.3.1.41 placenta low level Homo sapiens
-
2.3.1.41 skeletal muscle most abundant Homo sapiens
-
2.3.1.41 spleen low level Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.39 0.1
-
recombinant enzyme with recombinant ACP-A from Bacillus subtilis Homo sapiens
2.3.1.39 0.14
-
recombinant enzyme with recombinant human mitochondrial [acyl-carrier protein] lacking the first 68 amino acid residues Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + [acyl-carrier protein] absolutely specific for the substrates, type II [acyl-carrier protein] Homo sapiens CoA + malonyl-[acyl-carrier protein]
-
?
2.3.1.39 malonyl-CoA + [acyl-carrier protein] absolutely specific for the substrates, recombinant type II [acyl-carrier protein] lacking the N-terminal 68 amino acid residues from human, or recombinant ACP-A from Bacillus subtilis Homo sapiens CoA + malonyl-[acyl-carrier protein]
-
?
2.3.1.39 additional information enzyme is part of the malonyl-CoA-dependent type II fatty acid synthase complex in mitochondria Homo sapiens ?
-
?
2.3.1.41 acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Homo sapiens 3-oxobutanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 butanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Homo sapiens 3-oxohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Homo sapiens dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Homo sapiens 3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Homo sapiens 3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 additional information important role in generation of the lipoic acid precursor octanoyl-acyl-carrier protein Homo sapiens ?
-
?
2.3.1.41 additional information preference for acyl-[acyl-carrier protein] in decreasing order: C12, C10, C6, C8, C4, C2, C14, C16 Homo sapiens ?
-
?
2.3.1.41 myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Homo sapiens 3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Homo sapiens 3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
2.3.1.41 palmitoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Homo sapiens 3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.41 ? x * 45800, SDS-PAGE, recombinant protein without N-terminal targeting sequence, with His-tag Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.39 20
-
assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.41 6
-
myristoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 66
-
octanoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 91.2
-
butanoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 303
-
dodecanoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 353
-
hexanoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens
2.3.1.41 551
-
decanoyl-[acyl-carrier protein] pH 6.8, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 6.8
-
assay at Homo sapiens