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Literature summary extracted from

  • Berndt, C.; Lillig, C.H.; Wollenberg, M.; Bill, E.; Mansilla, M.C.; de Mendoza, D.; Seidler, A.; Schwenn, J.D.
    Characterization and reconstitution of a 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase from Bacillus subtilis (2004), J. Biol. Chem., 279, 7850-7855.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.8 gene cysH, expression in Escherichia coli strain BL21(DE3) Bacillus subtilis
1.8.99.2 expression in Escherichia coli of a tagged recombinant protein Bacillus subtilis

General Stability

EC Number General Stability Organism
1.8.4.8 activity is lost upon exposure to air or upon dilution Bacillus subtilis
1.8.99.2 unstable to air with a half-life of 12 min, the 4Fe-4S cluster is converted into a 2Fe-2S form Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.8 0.0064
-
 3'-phosphoadenylyl-sulfate recombinant enzyme, pH 8.0, with thioredoxin 1 Bacillus subtilis
1.8.4.8 0.0107
-
 3'-phosphoadenylyl-sulfate recombinant enzyme, pH 8.0, with glutaredoxin 1 Bacillus subtilis
1.8.4.8 0.0225
-
 glutaredoxin 1 recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate Bacillus subtilis
1.8.4.8 0.0287
-
 3'-phosphoadenylyl sulfate recombinant enzyme, pH 8.0, with thioredoxin 1 Bacillus subtilis
1.8.4.8 0.0543
-
 thioredoxin 1 recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate Bacillus subtilis
1.8.4.8 0.0606
-
 glutaredoxin 1 recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate Bacillus subtilis
1.8.4.8 0.0675
-
 thioredoxin 1 recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate Bacillus subtilis
1.8.4.8 0.1054
-
 3'-phosphoadenylyl sulfate recombinant enzyme, pH 8.0, with glutaredoxin 1 Bacillus subtilis
1.8.99.2 0.022
-
 reduced glutaredoxin 1 pH 8 and saturating adenylyl sulfate Bacillus subtilis
1.8.99.2 0.029
-
 adenylyl sulfate pH 8 and saturating thioredoxin 1 Bacillus subtilis
1.8.99.2 0.053
-
 thioredoxin 1 pH 8 and saturating adenylyl sulfate Bacillus subtilis
1.8.99.2 0.105
-
 adenylyl sulfate pH 8 and saturating glutaredoxin 1 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.4.8 Iron the enzyme contains a [4Fe-4S] cluster per subunit, absolutely required for activity, constitution is altered in presence of oxygen, activity is restored after decomposition and reassembly Bacillus subtilis
1.8.99.2 Iron contains a cubane 4Fe-4S cluster Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.8 27000
-
 2 * 27000, SDS-PAGE Bacillus subtilis
1.8.99.2 27000
-
 2 * 27000, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.8 3'-adenylyl-sulfate + thioredoxin Bacillus subtilis
-
 adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
 ?
1.8.4.8 3'-phosphoadenylyl-sulfate + glutaredoxin Bacillus subtilis
-
 adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite
-
 ?
1.8.4.8 3'-phosphoadenylyl-sulfate + thioredoxin Bacillus subtilis
-
 adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.8 Bacillus subtilis
-
 gene cysH, bispecific enzyme
-
1.8.99.2 Bacillus subtilis
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.8.4.8 activity is lost upon exposure to air Bacillus subtilis
1.8.99.2 oxygen causes complete loss of activity Bacillus subtilis

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.99.2 purification of a recombinant tagged protein by chitin affinity chromatography Bacillus subtilis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.8.99.2 the inactive 2Fe-2S form can be converted into the 4Fe-4S form by adding an iron-sulfur cluster synthesizing-protein from Synechocystis, restoring the activity Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.8 additional information
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.8 3'-adenylyl-sulfate + thioredoxin
-
 Bacillus subtilis adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
 ?
1.8.4.8 3'-phosphoadenylyl sulfate + glutaredoxin 1
-
 Bacillus subtilis adenosine 3',5'-bisphosphate + sulfite + glutaredoxin 1 disulfide
-
 r
1.8.4.8 3'-phosphoadenylyl sulfate + thioredoxin 1
-
 Bacillus subtilis adenosine 3',5'-bisphosphate + sulfite + thioredoxin 1 disulfide
-
 r
1.8.4.8 3'-phosphoadenylyl-sulfate + glutaredoxin
-
 Bacillus subtilis adenosine 3',5'-bisphosphate + glutaredoxin disulfide + sulfite
-
 ?
1.8.4.8 3'-phosphoadenylyl-sulfate + thioredoxin
-
 Bacillus subtilis adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
 ?
1.8.99.2 adenylyl sulfate + reduced glutaredoxin 1 can use 3'-phosphoadenylyl sulfate as sulfate donor Bacillus subtilis AMP + sulfite + oxidized glutaredoxin 1
-
 ?
1.8.99.2 adenylyl sulfate + reduced thioredoxin 1 can use 3'-phosphoadenylyl sulfate as sulfate donor Bacillus subtilis AMP + sulfite + oxidized thioredoxin 1
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.8 dimer 2 * 27000, SDS-PAGE Bacillus subtilis
1.8.99.2 homodimer 2 * 27000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.8.4.8 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase
-
 Bacillus subtilis
1.8.4.8 CysH1
-
 Bacillus subtilis
1.8.4.8 SNR
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.4.8 0.146
-
 glutaredoxin 1 recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate Bacillus subtilis
1.8.4.8 0.149
-
 3'-phosphoadenylyl sulfate recombinant enzyme, pH 8.0, with thioredoxin 1 Bacillus subtilis
1.8.4.8 0.172
-
 3'-phosphoadenylyl-sulfate recombinant enzyme, pH 8.0, with thioredoxin 1 Bacillus subtilis
1.8.4.8 0.174
-
 thioredoxin 1 recombinant enzyme, pH 8.0, with substrate 3'-adenylyl-sulfate Bacillus subtilis
1.8.4.8 0.219
-
 thioredoxin 1 recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate Bacillus subtilis
1.8.4.8 0.335
-
 3'-phosphoadenylyl sulfate recombinant enzyme, pH 8.0, with glutaredoxin 1 Bacillus subtilis
1.8.4.8 0.382
-
 3'-phosphoadenylyl-sulfate recombinant enzyme, pH 8.0, with glutaredoxin 1 Bacillus subtilis
1.8.4.8 0.421
-
 glutaredoxin 1 recombinant enzyme, pH 8.0, with substrate 3'-phosphoadenylyl-sulfate Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.8 8
-
 assay at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.8 glutaredoxin glutaredoxin 1 variant Bacillus subtilis
1.8.4.8 thioredoxin thioredoxin 1 variant Bacillus subtilis