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Literature summary extracted from

  • Graziani, S.; Xia, Y.; Gurnon, J.R.; Van Etten, J.L.; Leduc, D.; Skouloubris, S.; Myllykallio, H.; Liebl, U.
    Functional analysis of FAD-dependent thymidylate synthase ThyX from Paramecium bursaria Chlorella virus-1 (2004), J. Biol. Chem., 279, 54340-54347.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.148 expression in Escherichia coli Paramecium bursaria Chlorella virus-1

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.148 E190G no detectable activity Paramecium bursaria Chlorella virus-1

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.148 10-methyl-5,8-dideazafolate 0.2 mM, 90% inhibition Paramecium bursaria Chlorella virus-1
2.1.1.148 10-propargylfolate 0.2 mM, 85% inhibition Paramecium bursaria Chlorella virus-1
2.1.1.148 5-fluorodeoxyuridine 0.05 mM, 95% inhibition Paramecium bursaria Chlorella virus-1
2.1.1.148 8-aminopurinone deoxyribonucleoside 5'-phosphate 0.05 mM, 50% inhibition Paramecium bursaria Chlorella virus-1
2.1.1.148 additional information no inhibition by 10-propargyl-5,6-dideazafolate, 10-methylfolate, 8-azapurinone deoxyribonucleoside 5-phosphate Paramecium bursaria Chlorella virus-1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.148 0.015
-
 dUMP
-
 Paramecium bursaria Chlorella virus-1
2.1.1.148 0.02
-
 5,10-methylenetetrahydrofolate
-
 Paramecium bursaria Chlorella virus-1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.148 26000
-
 x * 26000, SDS-PAGE Paramecium bursaria Chlorella virus-1

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.148 Paramecium bursaria Chlorella virus-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.148
-
 Paramecium bursaria Chlorella virus-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Paramecium bursaria Chlorella virus-1 dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 additional information oxidation of NAD(P)H is linked to the reduction of enzyme bound FAD, dUMP is required for efficient FAD reduction, omitting of 5,10-methylenetetrahydrofolate from reaction mixture increases oxidation activity by a factor of 7 Paramecium bursaria Chlorella virus-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.148 ? x * 26000, SDS-PAGE Paramecium bursaria Chlorella virus-1

Synonyms

EC Number Synonyms Comment Organism
2.1.1.148 thymidylate synthase ThyX
-
 Paramecium bursaria Chlorella virus-1
2.1.1.148 ThyX
-
 Paramecium bursaria Chlorella virus-1

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.148 FAD noncovalently bound Paramecium bursaria Chlorella virus-1
2.1.1.148 NADH oxidation of NADH is linked to the reduction of enzyme bound FAD, dUMP is required for efficient FAD reduction, omitting of 5,10-methylenetetrahydrofolate from reaction mixture increases oxidation activity by a factor of 7 Paramecium bursaria Chlorella virus-1
2.1.1.148 NADPH oxidation of NADPH is linked to the reduction of enzyme bound FAD, dUMP is required for efficient FAD reduction, omitting of 5,10-methylenetetrahydrofolate from reaction mixture increases oxidation activity by a factor of 7 Paramecium bursaria Chlorella virus-1