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Literature summary extracted from

  • Koles, K.; Irvine, K.D.; Panin, V.M.
    Functional characterization of Drosophila sialyltransferase (2004), J. Biol. Chem., 279, 4346-4357.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.1 a full-length Drosophila SiaT protein with a C-terminal HA tag is transiently expressed in Drosophila S2 cells. A fraction of SiaT-HA protein expressed in S2 cell culture is secreted into the cell medium in soluble form Drosophila sp. (in: flies)

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.1 Co2+
-
 Drosophila sp. (in: flies)
2.4.3.1 Cu2+
-
 Drosophila sp. (in: flies)
2.4.3.1 Ni2+
-
 Drosophila sp. (in: flies)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.1 additional information
-
 additional information
-
 Drosophila sp. (in: flies)
2.4.3.1 1.4
-
 Galbeta(1-4)GlcNAc non-reducing terminus of asialofetuin glycans Drosophila sp. (in: flies)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.1 Golgi apparatus
-
 Drosophila sp. (in: flies) 5794
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.1 Drosophila sp. (in: flies)
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.3.1 sialoprotein recombinant Drosophila SiaT expressed in cultured insect cells possesses in vivo and in vitro autosialylation activity toward beta-linked GalNAc termini of its own N-linked glycans Drosophila sp. (in: flies)

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.1
-
 Drosophila sp. (in: flies)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.1 central nervous system expressed during embryonic development in a tissue- and stage-specific fashion, with elevated expression in a subset of cells within the central nervous system Drosophila sp. (in: flies)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetyl-beta-D-glucosamine 36% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Drosophila sp. (in: flies) CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,3-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 Drosophila sp. (in: flies) CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine-R exhibits highest activity toward GalNAcbeta1,4GlcNAc carbohydrate structures at the non-reducing termini of oligosaccharides and glycoprotein glycans. Oligosaccharides are preferred over glycoproteins as acceptors, and no activity toward glycolipid acceptors is detected. Recombinant Drosophila SiaT expressed in cultured insect cells possesses in vivo and in vitro autosialylation activity toward beta-linked GalNAc termini of its own N-linked glycans Drosophila sp. (in: flies) CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine-R
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + Galbeta(1-3)-GlcNAcbeta(1-3)Galbeta(1-4)Glc 30% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Drosophila sp. (in: flies) CMP + NeuNAcalpha(2-6)-Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc 79% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine Drosophila sp. (in: flies) CMP + alpha-N-acetylneuraminyl-(2-6)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 ?
2.4.3.1 CMP-N-acetylneuraminate + GalNAcbeta1,4-GlcNAc-R
-
 Drosophila sp. (in: flies) CMP + alpha-N-acetylneuraminyl-2,6-GalNAcbeta1,4-GlcNAc-R 190% of the activity with beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine ?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.1 SiaT
-
 Drosophila sp. (in: flies)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.3.1 25 37 activity at 25°C is about 75% of the activity at 100°C Drosophila sp. (in: flies)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.1 6 6.5
-
 Drosophila sp. (in: flies)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.3.1 5 7.5 about 40% of maximal activity at pH 5.0 and at pH 7.5 Drosophila sp. (in: flies)