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Literature summary extracted from

  • Armengaud, J.; Urbonavicius, J.; Fernandez, B.; Chaussinand, G.; Bujnicki, J.M.; Grosjean, H.
    N2-Methylation of guanosine at position 10 in tRNA is catalyzed by a THUMP domain-containing, S-adenosylmethionine-dependent methyltransferase, conserved in archaea and eukaryota (2004), J. Biol. Chem., 279, 37142-37152.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.213 overexpression in Escherichia coli Pyrococcus abyssi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.213 39000
-
 1 * 39000, SDS-PAGE Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.213 2 S-adenosyl-L-methionine + guanine10 in tRNA Pyrococcus abyssi depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures 2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA
-
 ?
2.1.1.213 S-adenosyl-L-methionine + guanine10 in tRNA Pyrococcus abyssi depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.213 Pyrococcus abyssi Q9UY84 strain GE5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.213
-
 Pyrococcus abyssi

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.213 S-adenosyl-L-methionine + guanine10 in tRNA = S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA (1a) Pyrococcus abyssi
a
2.1.1.213 S-adenosyl-L-methionine + N2-methylguanine10 in tRNA = S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA (1b) Pyrococcus abyssi
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.213 2 S-adenosyl-L-methionine + guanine10 in tRNA depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures Pyrococcus abyssi 2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA
-
 ?
2.1.1.213 S-adenosyl-L-methionine + guanine10 in tRNA depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures Pyrococcus abyssi S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.213 monomer 1 * 39000, SDS-PAGE Pyrococcus abyssi

Synonyms

EC Number Synonyms Comment Organism
2.1.1.213 N(2),N(2)-dimethylguanosine tRNA methyltransferase
-
 Pyrococcus abyssi
2.1.1.213 PAB1283
-
 Pyrococcus abyssi
2.1.1.213 PabTrm-G10
-
 Pyrococcus abyssi
2.1.1.213 Trm-G10
-
 Pyrococcus abyssi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.213 50
-
 assay at Pyrococcus abyssi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.213 86
-
 noticeable unfolding of the protein (more than 5%) is detected above 86°C Pyrococcus abyssi
2.1.1.213 102
-
 midpoint melting temperature Pyrococcus abyssi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.213 7.2
-
 assay at Pyrococcus abyssi