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Literature summary extracted from

  • Ketcham, C.; Wang, F.; Fisher, S.Z.; Ercan, A.; van der Wel, H.; Locke, R.D.; Sirajud-Doulah, k.; Matta, K.L.; West, C.M.
    Specificity of a soluble UDP-galactose:fucoside alpha1,3-galactosyltransferase that modifies the cytoplasmic glycoprotein Skp1 in Dictyostelium (2004), J. Biol. Chem., 279, 29050-29059.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.37 DTT required, best at 2 mM Dictyostelium discoideum
2.4.1.37 additional information not affected by Tween 20 at 0.1-1.0% Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.37 EDTA complete inhibition Dictyostelium discoideum
2.4.1.37 Fucalpha(1,2)Galbeta1-benzyl competitive, 37% and 61% inhibition at 1 mM and 4 mM, respectively Dictyostelium discoideum
2.4.1.37 additional information not affected by Tween 20 at 0.1-1.0% Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.37 additional information
-
additional information kinetics, hyperbolic dependence on UDP-Gal concentration Dictyostelium discoideum
2.4.1.37 0.0035
-
UDP-Gal pH 7.4, 22°C Dictyostelium discoideum
2.4.1.37 0.006
-
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-Skp1 protein pH 7.4, 22°C Dictyostelium discoideum
2.4.1.37 0.82
-
Fucalpha(1,2)Galbeta-1-(4-nitrophenol) pH 7.4, 22°C Dictyostelium discoideum
2.4.1.37 0.84
-
Fucalpha(1,2)Galbeta1-benzyl pH 7.4, 22°C Dictyostelium discoideum
2.4.1.37 1.2
-
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-(4-nitrophenol) pH 7.4, 22°C Dictyostelium discoideum
2.4.1.37 2.2
-
Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-benzyl pH 7.4, 22°C Dictyostelium discoideum
2.4.1.37 3.7
-
Fucalpha-1-(4-nitrophenol) pH 7.4, 22°C Dictyostelium discoideum
2.4.1.37 3.8
-
Fucalpha-1-benzyl pH 7.4, 22°C Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.37 cytoplasm
-
Dictyostelium discoideum 5737
-
2.4.1.37 additional information no activity in microsomes Dictyostelium discoideum
-
-
2.4.1.37 soluble
-
Dictyostelium discoideum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.37 KCl lower activity compared to NaCl, best at 50 mM Dictyostelium discoideum
2.4.1.37 Mg2+ 4fold lower activity compared to Mn2+ Dictyostelium discoideum
2.4.1.37 Mn2+ required, best at 2mM Dictyostelium discoideum
2.4.1.37 NaCl preferred salt, best at 50 mM Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.37 UDP-D-galactose + fucosylated Skp1 protein Dictyostelium discoideum
-
UDP + alpha-D-galactosyl(1-3)-fucosyl-Skp1 protein
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.37 Dictyostelium discoideum
-
Skp1B overexpressing strain HW302
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.37 native enzyme 2400fold by 2 steps ion exchange chromatography, HI chromatography, and gel filtration to homogeneity Dictyostelium discoideum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.37 additional information
-
-
Dictyostelium discoideum
2.4.1.37 72
-
purified native enzyme Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.37 additional information substrate specificity, enzyme forms Galalpha1,3Fuc-linkages, specific for L-fucose residues Dictyostelium discoideum ?
-
?
2.4.1.37 UDP-D-galactose + Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-(4-nitrophenol)
-
Dictyostelium discoideum UDP + alpha-D-Gal-1,3-Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-(4-nitrophenol)
-
?
2.4.1.37 UDP-D-galactose + Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-benzyl
-
Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-benzyl
-
?
2.4.1.37 UDP-D-galactose + Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-Skp1 protein best substrate, Skp1A1-(HW120)-myc and Skp1 from strain HL250 Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta(1,3)GlcNAcalpha-1-Skp1 protein
-
?
2.4.1.37 UDP-D-galactose + Fucalpha(1,2)Galbeta-1-(4-nitrophenol)
-
Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta-1-(4-nitrophenol)
-
?
2.4.1.37 UDP-D-galactose + Fucalpha(1,2)Galbeta-1-benzyl
-
Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha(1,2)Galbeta-1-benzyl
-
?
2.4.1.37 UDP-D-galactose + Fucalpha-1-(4-nitrophenol)
-
Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha-1-(4-nitrophenol)
-
?
2.4.1.37 UDP-D-galactose + Fucalpha-1-benzyl
-
Dictyostelium discoideum UDP + alpha-D-Gal(1,3)Fucalpha-1-benzyl
-
?
2.4.1.37 UDP-D-galactose + fucosylated Skp1 protein
-
Dictyostelium discoideum UDP + alpha-D-galactosyl(1-3)-fucosyl-Skp1 protein
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.37 alphaGal-T1
-
Dictyostelium discoideum
2.4.1.37 UDP-galactose:fucoside alpha1,3-galactosyltransferase
-
Dictyostelium discoideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.37 27
-
-
Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.37 6.4 7.4 dependent on the buffer system Dictyostelium discoideum