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Literature summary extracted from

  • Levitan, A.; Danon, A.; Lisowsky, T.
    Unique features of plant mitochondrial sulfhydryl oxidase (2004), J. Biol. Chem., 279, 20002-20008.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.2 gene AtErv1, DNA and amino acid sequence analysis, subcloning in Escherichia coli strain DH5alpha, expression of full length enzyme and 15 kDa C-terminal fragment as His-tagged proteins in Escherichia coli strain BL21, transient expression of the enzyme fused to GFP in protoplasts of Arabidopsis thaliana and of Physcomitrella patens with localization in the mitochondria, no complementation of the enzyme-defective yeast mutant erv-ts and yeast deletion mutant DELTAerv1 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.3.2 mitochondrion preferentially Arabidopsis thaliana 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.2 22000
-
 x * 22000, full length enzyme, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.3.2 R-SH + O2 Arabidopsis thaliana enzyme is essential for biogenesis of mitochondrial and cytosolic iron sulfur cluster assembly R-S-S-R + H2O2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.2 Arabidopsis thaliana Q8GXX0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.2 recombinant His-tagged full length enzyme and C-terminal fragment from Escherichia coli strain BL21 to homogeneity by nickel affinity chromatography Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.3.2 2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2 enzyme contains a functionally involved redox-active motif YPCCXXC Arabidopsis thaliana
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.2 cysteine + O2 artificial in vitro substrate Arabidopsis thaliana cystine + H2O2
-
 ir
1.8.3.2 dithiothreitol + O2 artificial in vitro substrate Arabidopsis thaliana dithiothreitol disulfide + H2O2
-
 ir
1.8.3.2 additional information no activity with glutathione, 2-mercaptoethanol, and di(2-mercaptoethanol) Arabidopsis thaliana ?
-
 ?
1.8.3.2 R-SH + O2
-
 Arabidopsis thaliana R-S-S-R + H2O2
-
 ir
1.8.3.2 R-SH + O2 enzyme is essential for biogenesis of mitochondrial and cytosolic iron sulfur cluster assembly Arabidopsis thaliana R-S-S-R + H2O2
-
 ir
1.8.3.2 thioredoxin + O2
-
 Arabidopsis thaliana thioredoxin disulfide + H2O2
-
 ir

Subunits

EC Number Subunits Comment Organism
1.8.3.2 ? x * 22000, full length enzyme, SDS-PAGE Arabidopsis thaliana
1.8.3.2 More structural analysis, modeling of the conserved central domain, the plant enzyme contains a unique C-terminally located CXXXXC motif and no N-terminally localized cysteine pair, which is typical for enzymes of the Erv1/Alr sulfhydryl oxidase family Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.8.3.2 Erv1
-
 Arabidopsis thaliana
1.8.3.2 More the enzyme belongs to the Erv1/Alr sulfhydryl oxidase family Arabidopsis thaliana
1.8.3.2 sulfhydryl oxidase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.3.2 25
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.2 7.5
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.3.2 FAD noncovalently bound, binding site sequence and structure Arabidopsis thaliana