EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.3.1 | Y343F | in the mutant enzyme using cytochrome c as electron acceptor, turnover number is somewhat impaired, 34% of the wild-type activity at pH 8.5. The KM-value for the mutant enzyme shows a 5fold increase over wild-type. Reduction of the molybdenum center of the Y343 F variant by sulfite is more significantly impaired at high pH than at low pH | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.1 | 0.00129 | - |
sulfite | 25°C, pH 6.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00162 | - |
sulfite | 25°C, pH 6.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00272 | - |
sulfite | 25°C, pH 7.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00311 | - |
sulfite | 25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00339 | - |
sulfite | 25°C, pH 7.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00352 | - |
sulfite | 25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00362 | - |
sulfite | 25°C, pH 7.0, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00367 | - |
sulfite | 25°C, pH 7.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00414 | - |
sulfite | 25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00423 | - |
sulfite | 25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00435 | - |
sulfite | 25°C, pH 8.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00459 | - |
sulfite | 25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00503 | - |
sulfite | 25°C, pH 8.25, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00612 | - |
sulfite | 25°C, pH 8.0, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00635 | - |
sulfite | 25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00728 | - |
sulfite | 25°C, pH 8.25, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00825 | - |
sulfite | 25°C, pH 8.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00851 | - |
sulfite | 25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00859 | - |
sulfite | 25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00908 | - |
sulfite | 25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00924 | - |
sulfite | 25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00947 | - |
sulfite | 25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00959 | - |
sulfite | 25°C, pH 8.75, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.00963 | - |
sulfite | 25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.00992 | - |
sulfite | 25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.011 | - |
sulfite | 25°C, pH 8.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.0158 | - |
sulfite | 25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.0221 | - |
sulfite | 25°C, pH 9.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.026 | - |
sulfite | 25°C, pH 9.0, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.0319 | - |
sulfite | 25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.0536 | - |
sulfite | 25°C, pH 9.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.0557 | - |
sulfite | 25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.0671 | - |
sulfite | 25°C, pH 9.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 0.147 | - |
sulfite | 25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 0.59 | 1 | sulfite | 25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 1.54 | - |
sulfite | 25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.3.1 | Homo sapiens | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.3.1 | sulfite + O2 + H2O | - |
Homo sapiens | sulfate + H2O2 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.3.1 | 2.06 | - |
sulfite | 25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 3.11 | - |
sulfite | 25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 3.26 | - |
sulfite | 25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 4.14 | - |
sulfite | 25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 4.59 | - |
sulfite | 25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 6.35 | - |
sulfite | 25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 7.17 | - |
sulfite | 25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 8.21 | - |
sulfite | 25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 8.51 | - |
sulfite | 25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 8.59 | - |
sulfite | 25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 8.72 | - |
sulfite | 25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 9.08 | - |
sulfite | 25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 9.2 | - |
sulfite | 25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 9.24 | - |
sulfite | 25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 9.47 | - |
sulfite | 25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 9.63 | - |
sulfite | 25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 9.92 | - |
sulfite | 25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 9.99 | - |
sulfite | 25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 10.5 | - |
sulfite | 25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.8.3.1 | 12.1 | - |
sulfite | 25°C, pH 7.0, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 13.2 | - |
sulfite | 25°C, pH 6.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 17.2 | - |
sulfite | 25°C, pH 7.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 17.7 | - |
sulfite | 25°C, pH 6.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 23.6 | - |
sulfite | 25°C, pH 8.75, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 24.2 | - |
sulfite | 25°C, pH 7.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 24.6 | - |
sulfite | 25°C, pH 9.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 24.7 | - |
sulfite | 25°C, pH 7.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 24.8 | - |
sulfite | 25°C, pH 8.25, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 25 | - |
sulfite | 25°C, pH 8.0, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 25.7 | - |
sulfite | 25°C, pH 9.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 25.9 | - |
sulfite | 25°C, pH 8.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 26.3 | - |
sulfite | 25°C, pH 9.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 26.9 | - |
sulfite | 25°C, pH 8.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 26.9 | - |
sulfite | 25°C, pH 8.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 27 | - |
sulfite | 25°C, pH 8.25, 100 mM buffer, wild-type enzyme | Homo sapiens | |
1.8.3.1 | 28.1 | - |
sulfite | 25°C, pH 9.0, 100 mM buffer, wild-type enzyme | Homo sapiens |