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Literature summary extracted from

  • Baker, L.M.S.; Poole, L.B.
    Catalytic mechanism of thiol peroxidase from Escherichia coli: Sulfenic acid formation and overoxidation of essential Cys61 (2003), J. Biol. Chem., 278, 9203-9211.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.7 overexpression in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.7 C61S no activity Escherichia coli
1.11.1.7 C82S 72% of wild-type activity Escherichia coli
1.11.1.7 C82S/C95S 10% of wild-type activity Escherichia coli
1.11.1.7 C95S 20% of wild-type activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.009
-
 cumene hydroperoxide 25°C, pH 7.0 Escherichia coli
1.11.1.7 0.023
-
 thioredoxin 1 25°C, pH 7.0 Escherichia coli
1.11.1.7 1.7
-
 H2O2 25°C, pH 7.0 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 17700
-
 2 * 17700, ESI-mass spectrometry, dimer in solution Escherichia coli
1.11.1.7 29500
-
 sedimentation equilibrium, oxidized enzyme Escherichia coli
1.11.1.7 32000
-
 sedimentation equilibrium, reduced enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Escherichia coli P0A862
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 ammonium sulfate, Phenyl-Sepharodse, Q-Sepharose Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 reduced thioredoxin 1 + cumene hydroperoxide
-
 Escherichia coli oxidized thioredoxin 1 + cumene hydroxide
-
 ?
1.11.1.7 reduced thioredoxin 1 + H2O2
-
 Escherichia coli oxidized thioredoxin 1 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.7 dimer 2 * 17700, ESI-mass spectrometry, dimer in solution Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 p20
-
 Escherichia coli
1.11.1.7 scavengase
-
 Escherichia coli
1.11.1.7 thiol peroxidase
-
 Escherichia coli
1.11.1.7 Tpx
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.7 70.1
-
 cumene hydroperoxide 25°C, pH 7.0 Escherichia coli
1.11.1.7 76
-
 H2O2 25°C, pH 7.0 Escherichia coli