EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.4.11 | expression of wild-type and mutant MsrAs in Escherichia coli strain BE002 | Neisseria meningitidis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.4.11 | C198S | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme | Neisseria meningitidis |
1.8.4.11 | C198S | site-directed mutagenesis, altered kinetics and disulfide bond formation compared to the wild-type enzyme | Neisseria meningitidis |
1.8.4.11 | W35F | site-directed mutagenesis, altered kinetics and disulfide bond formation compared to the wild-type enzyme | Neisseria meningitidis |
1.8.4.11 | W53F | site-directed mutagenesis, altered kinetics compared to the wild-type enzyme | Neisseria meningitidis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.11 | additional information | - |
additional information | kinetics of disulfide formation in MsrA at Cys52/Cys198 at pH 5.5 and pH 8.0, 25°C, single turnover experiments, steady-state kinetics | Neisseria meningitidis | |
1.8.4.11 | additional information | - |
additional information | detailed kinetics, wild-type and mutants MsrAs | Neisseria meningitidis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.4.11 | Neisseria meningitidis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.4.11 | recombinant wild-type and mutant MsrAs from Escherichia coli strain BE002 | Neisseria meningitidis |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O | catalytic mechanism of MsrA, the rate limiting step occurs after formation of the sulfenic acid intermediate and is associated with either the Cys51/Cys198 disulfide bond formation or the thioredoxin reduction process | Neisseria meningitidis | |
1.8.4.11 | peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin | catalytic mechanism, rate-limiting reduction of the Cys51-Cys198 disulfide bond by thioredoxin and formation of the thiosulfenic acid intermediate on Cys51 | Neisseria meningitidis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | enzyme MsrA shows absolute specificity for the S-form of free methionine sulfoxide, no activity with the R-form, enzyme MsrA is oxidized at Cys51/Cys198 forming a disulfide | Neisseria meningitidis | L-methionine + thioredoxin disulfide + H2O | - |
? | |
1.8.4.11 | L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction | Neisseria meningitidis | L-methionine + thioredoxin disulfide + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.4.11 | methionine sulfoxide reductase A | - |
Neisseria meningitidis |
1.8.4.11 | MsrA | - |
Neisseria meningitidis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.4.11 | 25 | - |
assay at | Neisseria meningitidis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.4.11 | additional information | - |
additional information | - |
Neisseria meningitidis | |
1.8.4.11 | 0.6 | - |
L-methionine (S)-sulfoxide | pH 5.5, 25°C, MsrA, steady-state conditions | Neisseria meningitidis | |
1.8.4.11 | 7 | - |
L-methionine (S)-sulfoxide | pH 8.0, 25°C, MsrA, steady-state conditions | Neisseria meningitidis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.4.11 | 5.5 | - |
assay at | Neisseria meningitidis |
1.8.4.11 | 8 | - |
L-methionine formation | Neisseria meningitidis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.8.4.11 | 5.5 | 8 | - |
Neisseria meningitidis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.4.11 | thioredoxin | - |
Neisseria meningitidis | |
1.8.4.11 | thioredoxin | rate-limiting reduction of the Cys51-Cys198 disulfide bond by thioredoxin in catalysis | Neisseria meningitidis |