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Literature summary extracted from

  • Angelaccio, S.; Chiaraluce, R.; Consalvi, V.; Buchenau, B.; Giangiacomo, L.; Bossa, F.; Contestabile, R.
    Catalytic and thermodynamic properties of tetrahydromethanopterin-dependent serine hydroxymethyltransferase from Methanococcus jannaschii (2003), J. Biol. Chem., 278, 41789-41797.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expression in Escherichia coli Methanocaldococcus jannaschii

General Stability

EC Number General Stability Organism
2.1.2.1 above 6 M urea the dichrpoic activity is reduced significantly with a transition midpoint at 2.56 M. This process is completely reversible Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.1
-
 tetrahydromethanopterin pH 7.4, 37°C Methanocaldococcus jannaschii
2.1.2.1 0.8
-
 L-Ser pH 7.4, 37°C Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 48200
-
 2 * 48200, SDS-PAGE Methanocaldococcus jannaschii
2.1.2.1 98000
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 recombinant enzyme Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 tetrahydromethanopterin + L-Ser
-
 Methanocaldococcus jannaschii glycine + ?
-
 ?
2.1.2.1 tetrahydropteroylglutamate + L-Ser
-
 Methanocaldococcus jannaschii glycine + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 dimer 2 * 48200, SDS-PAGE Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 80
-
 retroaldol cleavage of L-allo-threonine Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 3.53
-
 L-Ser pH 7.4, 37°C Methanocaldococcus jannaschii
2.1.2.1 3.53
-
 tetrahydromethanopterin pH 7.4, 37°C Methanocaldococcus jannaschii