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Literature summary extracted from

  • El Alami, M.; Dubois, E.; Oudjama, Y.; Tricot, C.; Wouters, J.; Stalon, V.; Messenguy, F.
    Yeast epiarginase regulation, an enzyme-enzyme activity control: identification of residues of L-ornithine carbamoyltransferase and arginase responsible for enzyme catalytic and regulatory activities (2003), J. Biol. Chem., 278, 21550-21558.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.3 D182N greatly reduced affinity for L-ornithine, no interaction with arginase Saccharomyces cerevisiae
2.1.3.3 E123A little change in activity, or in sensitivity to arginase Saccharomyces cerevisiae
2.1.3.3 E123S little change in activity, or in sensitivity to arginase Saccharomyces cerevisiae
2.1.3.3 E256A greatly reduced affinity for L-ornithine, impaired interaction with arginase Saccharomyces cerevisiae
2.1.3.3 E256Q greatly reduced affinity for L-ornithine, impaired interaction with arginase Saccharomyces cerevisiae
2.1.3.3 G181R no residual activity Saccharomyces cerevisiae
2.1.3.3 K260A important in mediating sensitivity to L-ornithine and arginase Saccharomyces cerevisiae
2.1.3.3 K260R important in mediating sensitivity to L-ornithine and arginase Saccharomyces cerevisiae
2.1.3.3 K263R important in mediating sensitivity to L-ornithine and arginase Saccharomyces cerevisiae
2.1.3.3 K265A important in mediating sensitivity to L-ornithine and arginase Saccharomyces cerevisiae
2.1.3.3 K265R important in mediating sensitivity to L-ornithine and arginase Saccharomyces cerevisiae
2.1.3.3 K268A important in mediating sensitivity to L-ornithine and arginase Saccharomyces cerevisiae
2.1.3.3 K268R important in mediating sensitivity to L-ornithine and arginase Saccharomyces cerevisiae
2.1.3.3 K289S greatly reduced affinity for L-ornithine, impaired interaction with arginase Saccharomyces cerevisiae
2.1.3.3 L290Q reduced activity, little change in sensitivity to arginase Saccharomyces cerevisiae
2.1.3.3 L290S reduced activity, little change in sensitivity to arginase Saccharomyces cerevisiae
2.1.3.3 N184Q greatly reduced affinity for L-ornithine, impaired interaction with arginase Saccharomyces cerevisiae
2.1.3.3 N185Q greatly reduced affinity for L-ornithine, impaired interaction with arginase Saccharomyces cerevisiae
2.1.3.3 Q294P little change in activity, or in sensitivity to arginase Saccharomyces cerevisiae
2.1.3.3 T68G greatly reduced activity, no change in sensitivity to arginase Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 arginase 500 units, 14% remaining activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.3 0.05
-
 L-ornithine mutant K268A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 1
-
 L-ornithine mutant K260A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 1.1
-
 L-ornithine mutant K265R, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 1.1
-
 L-ornithine mutant K268R, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 1.2
-
 L-ornithine mutant E256Q, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 1.2
-
 L-ornithine mutant K260R, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 1.2
-
 L-ornithine mutant K265A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 1.3
-
 L-ornithine mutant Q294P, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 1.6
-
 L-ornithine wild-type, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 2
-
 L-ornithine mutant L290Q, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 2
-
 L-ornithine mutant L290S, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 2.1
-
 L-ornithine mutant E123S, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 2.6
-
 L-ornithine mutant E123A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 2.8
-
 L-ornithine mutant C191M/C194N/F197V, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 3.4
-
 L-ornithine mutant T68G, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 10
-
 L-ornithine mutant E256A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 10
-
 L-ornithine mutant K289S, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 20
-
 L-ornithine mutant K263R, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 30
-
 L-ornithine mutant K263A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 34
-
 L-ornithine mutant N184Q, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 49
-
 L-ornithine mutant D182N, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 350
-
 L-ornithine mutant N185Q, pH 8.0, 30°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.3 1.28
-
 mutant T68G, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 2.83
-
 mutant K289S, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 4.16
-
 mutant C191M/C194N/F197V, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 4.66
-
 mutant L290S, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 7.5
-
 mutant K263A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 12.33
-
 mutant N184Q, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 12.66
-
 mutant E256Q, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 13.3
-
 mutant L290Q, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 14.83
-
 mutant K263R, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 15
-
 mutant E256A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 16.16
-
 mutant D182N, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 16.83
-
 mutant K265A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 23.38
-
 mutant E123S, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 23.63
-
 mutant K265R, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 25
-
 mutant K268A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 25
-
 mutant N185Q, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 25.16
-
 mutant K260R, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 25.3
-
 mutant E123A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 25.73
-
 mutant K268R, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 25.83
-
 mutant K260A, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 26.12
-
 wild-type, pH 8.0, 30°C Saccharomyces cerevisiae
2.1.3.3 48.66
-
 mutant Q294P, pH 8.0, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Saccharomyces cerevisiae phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 More in presence of ornitthine and arginine, enzyme forms a complex with arginase of 200 kDa Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.1.3.3 otcase
-
 Saccharomyces cerevisiae