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Literature summary extracted from

  • Savvides, S.N.; Scheiwein, M.; Bohme, C.C.; Arteel, G.E.; Karplus, P.A.; Becker, K.; Schirmer, R.H.
    Crystal structure of the antioxidant enzyme glutathione reductase inactivated by peroxynitrite (2002), J. Biol. Chem., 277, 2779-2784.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.1.7 peroxynitrite inactivated enzyme, hanging drop vapour dffusion method, 10 mg/ml protein in 120 mM ammonium sulfate, 100 mM potassium phosphate, pH 7.5, against reservoir solution containing 720 mM ammonium sulfate, 100 mM potassium phosphate, pH 8.0, 30-37°C, 5 days, storage and handling in stabilization solution containing 1 M ammonium sulfate, 100 mM potassium phosphate, pH 8.0, X-ray structure determination and analysis at 1.9 A resolution Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.7 Y114L site-directed mutagenesis, mutant is insensitive to inactivation by peroxynitrite in contrast to the wild-type enzyme Homo sapiens
1.8.1.7 Y114L site-directed mutagenesis, mutant is insensitive to inactivation by peroxynitrite in contratst to the wild-type enzyme Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.7 additional information bis-nitro-enzyme form is impaired in peptide substrate binding with a 20fold increased Km for glutathione disulfide, overview Homo sapiens
1.8.1.7 peroxynitrite inactivation by modification of Tyr114 and Tyr106 Homo sapiens
1.8.1.7 peroxynitrite inactivation by modification of Tyr114 and Tyr106 Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.072
-
 glutathione disulfide pH 7.4, 25°C Homo sapiens
1.8.1.7 0.095
-
 glutathione disulfide pH 7.4, 25°C Plasmodium falciparum
1.8.1.7 1.65
-
 glutathione disulfide mutant enzyme, pH 7.4, 25°C Homo sapiens
1.8.1.7 2.31
-
 glutathione disulfide mutant enzyme, pH 7.4, 25°C Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH Plasmodium falciparum
-
 glutathione + NADP+
-
 ?
1.8.1.7 glutathione disulfide + NADPH Homo sapiens
-
 glutathione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Homo sapiens P00390
-
-
1.8.1.7 Plasmodium falciparum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 additional information
-
-
 Plasmodium falciparum
1.8.1.7 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH
-
 Plasmodium falciparum glutathione + NADP+
-
 ?
1.8.1.7 glutathione disulfide + NADPH
-
 Homo sapiens glutathione + NADP+
-
 ?
1.8.1.7 glutathione disulfide + NADPH enzyme contains an active site disulfide/dithiol depending on the redox status Plasmodium falciparum glutathione + NADP+
-
 ?
1.8.1.7 glutathione disulfide + NADPH enzyme contains an active site disulfide/dithiol depending on the redox status Homo sapiens glutathione + NADP+
-
 ?
1.8.1.7 peroxynitrite + NADPH
-
 Plasmodium falciparum nitrite + H2O + NADP+
-
 ?
1.8.1.7 peroxynitrite + NADPH
-
 Homo sapiens nitrite + H2O + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 glutathione reductase
-
 Plasmodium falciparum
1.8.1.7 glutathione reductase
-
 Homo sapiens
1.8.1.7 GR
-
 Plasmodium falciparum
1.8.1.7 GR
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 25
-
 assay at Plasmodium falciparum
1.8.1.7 25
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.7 2
-
 glutathione disulfide mutant enzyme, pH 7.4, 25°C Plasmodium falciparum
1.8.1.7 5
-
 glutathione disulfide mutant enzyme, pH 7.4, 25°C Homo sapiens
1.8.1.7 120
-
 glutathione disulfide pH 7.4, 25°C Plasmodium falciparum
1.8.1.7 165
-
 glutathione disulfide pH 7.4, 25°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7.4
-
 assay at Plasmodium falciparum
1.8.1.7 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 NADPH
-
 Plasmodium falciparum
1.8.1.7 NADPH
-
 Homo sapiens