EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.135 | expression in Pichia pastoris | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.135 | H308A | mutation abrogates the activity towards UDP-GlcA | Homo sapiens |
2.4.1.135 | H308R | mutation induces a major change in specificity. In contrast to wild-type enzyme the mutant is able to efficiently transfer Glc from UDP-Glc onto acceptor substrate Galbeta(1-3)Gal. The mutant enzyme remains able to catalyze the transfer of GlcA from UDP-GlyA onto Galbeta(1-3)Gal. UDP-GlcNAc is used at about the same rate as UDP-Glc. UDP-Gal is a weak donor substrate. UDP-Man is efficiently used as cosubstrate. No activity with GDP-Man | Homo sapiens |
2.4.1.135 | H308R/R277A | mutant enzyme shows no activity with UDP-GlcA as donor substrate, mutant enzyme is active with UDP-Gly as donor | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.135 | 2,3-Butanedione | irreversible. UDP-glucuronic acid protects, no protection by UDP-glucose. Activity of H308R towards UDP-glucose is unaffected by the reagent | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.135 | 0.0499 | - |
UDP-GlcA | pH 5.0, 37°C, mutant enzyme H308R | Homo sapiens | |
2.4.1.135 | 0.059 | - |
UDP-GlcA | pH 5.0, 37°C, wild-type enzyme | Homo sapiens | |
2.4.1.135 | 0.074 | - |
UDP-Man | pH 5.0, 37°C, mutant enzyme H308R | Homo sapiens | |
2.4.1.135 | 0.108 | - |
UDP-GlcNAc | pH 5.0, 37°C, mutant enzyme H308R | Homo sapiens | |
2.4.1.135 | 0.233 | - |
UDP-glucose | pH 5.0, 37°C, mutant enzyme H308R | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.135 | additional information | Homo sapiens | enzyme plays a key role in proteoglycan biosynthesis by catalyzing the transfer of glucuronic acid onto the trisaccharide-protein linkage structure Galbeta(1-3)Galbeta(1-4)Xyl-O-Ser, a prerequisite step for polymerization of glycosaminoglycan chains | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.135 | Homo sapiens | Q9P2W7 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.135 | additional information | enzyme plays a key role in proteoglycan biosynthesis by catalyzing the transfer of glucuronic acid onto the trisaccharide-protein linkage structure Galbeta(1-3)Galbeta(1-4)Xyl-O-Ser, a prerequisite step for polymerization of glycosaminoglycan chains | Homo sapiens | ? | - |
? | |
2.4.1.135 | additional information | no activity with wild-type enzyme: UDP-Glc, IDP-Gal, UDP-Man and UDP-GlcNAc | Homo sapiens | ? | - |
? | |
2.4.1.135 | UDP-Gal + Galbeta(1-3)Gal | no activity with wild-type enzyme, weak activity with mutant enzyme H308R | Homo sapiens | Galbeta(1-3)Galbeta(1-3)Gal + UDP | - |
? | |
2.4.1.135 | UDP-galacturonic acid + Galbeta(1-3)Gal | - |
Homo sapiens | Galbeta(1-3)Galbeta(1-3)Gal + UDP | - |
? | |
2.4.1.135 | UDP-GlcNAc + Galbeta(1-3)Gal | no activity with wild-type enzyme, activity with mutant enzyme H308R is nearly equal to activity with UDP-Glc | Homo sapiens | GlcNAcbeta(1-3)Galbeta(1-3)Gal + UDP | - |
? | |
2.4.1.135 | UDP-glucose + galactosyl-1,3-thiogalactose | - |
Homo sapiens | ? | - |
? | |
2.4.1.135 | UDP-glucuronate + Galbeta(1-3)Gal | - |
Homo sapiens | GlcAbeta(1-3)Galbeta(1-3)Gal + UDP | - |
? | |
2.4.1.135 | UDP-Man + Galbeta(1-3)Gal | no activity with wild-type enzyme, efficient reaction with mutant enzyme H308R | Homo sapiens | Manbeta(1-3)Galbeta(1-3)Gal + UDP | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.135 | beta1,3-glucuronosyltransferase I | - |
Homo sapiens |
2.4.1.135 | GlcAT-I | - |
Homo sapiens |