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Literature summary extracted from

  • Ouzzine, M.; Gulberti, S.; Levoin, N.; Netter, P.; Magdalou, J.; Fournel-Gigleux, S.
    The donor substrate specificity of the human beta1,3-glucuronosyltransferase I toward UDP-glucuronic acid is determined by two crucial histidine and arginine residues (2002), J. Biol. Chem., 277, 25439-25445.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.135 expression in Pichia pastoris Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.135 H308A mutation abrogates the activity towards UDP-GlcA Homo sapiens
2.4.1.135 H308R mutation induces a major change in specificity. In contrast to wild-type enzyme the mutant is able to efficiently transfer Glc from UDP-Glc onto acceptor substrate Galbeta(1-3)Gal. The mutant enzyme remains able to catalyze the transfer of GlcA from UDP-GlyA onto Galbeta(1-3)Gal. UDP-GlcNAc is used at about the same rate as UDP-Glc. UDP-Gal is a weak donor substrate. UDP-Man is efficiently used as cosubstrate. No activity with GDP-Man Homo sapiens
2.4.1.135 H308R/R277A mutant enzyme shows no activity with UDP-GlcA as donor substrate, mutant enzyme is active with UDP-Gly as donor Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.135 2,3-Butanedione irreversible. UDP-glucuronic acid protects, no protection by UDP-glucose. Activity of H308R towards UDP-glucose is unaffected by the reagent Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.135 0.0499
-
UDP-GlcA pH 5.0, 37°C, mutant enzyme H308R Homo sapiens
2.4.1.135 0.059
-
UDP-GlcA pH 5.0, 37°C, wild-type enzyme Homo sapiens
2.4.1.135 0.074
-
UDP-Man pH 5.0, 37°C, mutant enzyme H308R Homo sapiens
2.4.1.135 0.108
-
UDP-GlcNAc pH 5.0, 37°C, mutant enzyme H308R Homo sapiens
2.4.1.135 0.233
-
UDP-glucose pH 5.0, 37°C, mutant enzyme H308R Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.135 additional information Homo sapiens enzyme plays a key role in proteoglycan biosynthesis by catalyzing the transfer of glucuronic acid onto the trisaccharide-protein linkage structure Galbeta(1-3)Galbeta(1-4)Xyl-O-Ser, a prerequisite step for polymerization of glycosaminoglycan chains ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.135 Homo sapiens Q9P2W7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.135 additional information enzyme plays a key role in proteoglycan biosynthesis by catalyzing the transfer of glucuronic acid onto the trisaccharide-protein linkage structure Galbeta(1-3)Galbeta(1-4)Xyl-O-Ser, a prerequisite step for polymerization of glycosaminoglycan chains Homo sapiens ?
-
?
2.4.1.135 additional information no activity with wild-type enzyme: UDP-Glc, IDP-Gal, UDP-Man and UDP-GlcNAc Homo sapiens ?
-
?
2.4.1.135 UDP-Gal + Galbeta(1-3)Gal no activity with wild-type enzyme, weak activity with mutant enzyme H308R Homo sapiens Galbeta(1-3)Galbeta(1-3)Gal + UDP
-
?
2.4.1.135 UDP-galacturonic acid + Galbeta(1-3)Gal
-
Homo sapiens Galbeta(1-3)Galbeta(1-3)Gal + UDP
-
?
2.4.1.135 UDP-GlcNAc + Galbeta(1-3)Gal no activity with wild-type enzyme, activity with mutant enzyme H308R is nearly equal to activity with UDP-Glc Homo sapiens GlcNAcbeta(1-3)Galbeta(1-3)Gal + UDP
-
?
2.4.1.135 UDP-glucose + galactosyl-1,3-thiogalactose
-
Homo sapiens ?
-
?
2.4.1.135 UDP-glucuronate + Galbeta(1-3)Gal
-
Homo sapiens GlcAbeta(1-3)Galbeta(1-3)Gal + UDP
-
?
2.4.1.135 UDP-Man + Galbeta(1-3)Gal no activity with wild-type enzyme, efficient reaction with mutant enzyme H308R Homo sapiens Manbeta(1-3)Galbeta(1-3)Gal + UDP
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.135 beta1,3-glucuronosyltransferase I
-
Homo sapiens
2.4.1.135 GlcAT-I
-
Homo sapiens