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Literature summary extracted from

  • Vickers, C.; Hales, P.; Kaushik, V.; Dick, L.; Gavin, J.; Tang, J.; Godbout, K.; Parsons, T.; Baronas, E.; Hsieh, F.; Acton, S.; Patane, M.; Nichols, A.; Tummino, P.
    Hydrolysis of biological peptides by human angiotensin-converting enzyme-related carboxypeptidase (2002), J. Biol. Chem., 277, 14838-14843.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.23 expression in Spodoptera frugiperda Sf9 cells via infection with baculovirus Homo sapiens

General Stability

EC Number General Stability Organism
3.4.17.23 Zn2+ stabilizes Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.23 0.147
-
 (7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH pH 6.5, room temperature Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.23 Cl- enhances activity by about 10fold Homo sapiens
3.4.17.23 F- enhances activity by about 10fold Homo sapiens
3.4.17.23 additional information no effect of Br- Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.23 89600
-
 recombinant enzyme, MALDI-TOF mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.23 angiotensin I + H2O Homo sapiens
-
 angiotensin-(1-9) + Leu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.23 Homo sapiens Q9BYF1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.23 recombinant from Sf9 cells, to near homogeneity Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.17.23 angiotensin II + H2O = angiotensin-(1-7) + L-phenylalanine ACE2 catalytic efficiency is 400-fold higher with angiotensin II (1–8) as a substrate than with angiotensin I (1–10). ACE2 also efficiently hydrolyzes des-Arg9-bradykinin, but it does not hydrolyze bradykinin Homo sapiens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.23 (7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH + H2O synthetic fluorogenic substrate Homo sapiens (7-methoxycoumarin-4-yl)acetyl-AP + N6-(2,4-dinitrophenyl)-L-lysine
-
 ?
3.4.17.23 (7-methoxycoumarin-4-yl)acetyl-YVADAPK(2,4-dinitrophenyl)-OH + H2O synthetic fluorogenic caspase-1 substrate Homo sapiens (7-methoxycoumarin-4-yl)acetyl-YVADAP + N6-(2,4-dinitrophenyl)-L-lysine
-
 ?
3.4.17.23 angiotensin I + H2O
-
 Homo sapiens angiotensin-(1-9) + Leu
-
 ?
3.4.17.23 angiotensin II + H2O 400fold higher activity than with angiotensin I Homo sapiens angiotensin-(1-7) + Phe
-
 ?
3.4.17.23 apelin-13 + H2O
-
 Homo sapiens apelin-12 + Phe
-
 ?
3.4.17.23 apelin-36 + H2O
-
 Homo sapiens apelin-35 + Phe
-
 ?
3.4.17.23 dynorphin A 1-13 + H2O
-
 Homo sapiens dynorphin A 1-12 + Lys
-
 ir
3.4.17.23 ghrelin + H2O
-
 Homo sapiens ghrelin minus C-terminal amino acid + arginine
-
 ir
3.4.17.23 KRPPGSPF + H2O i.e. Lys-des-Arg-bradykinin Homo sapiens KRPPGSP + Phe
-
 ir
3.4.17.23 neocasomorphin + H2O
-
 Homo sapiens neocasomorphin minus C-terminal amino acid + isoleucine
-
 ir
3.4.17.23 neurotensin-(1-8) + H2O
-
 Homo sapiens neurotensin-(1-7) + arginine
-
 ir
3.4.17.23 RPPGSPF + H2O i.e. des-Arg-bradykinin Homo sapiens RPPGSP + Phe i.e. des-Arg-bradykinin-(1-7) ir
3.4.17.23 YPVEPFI + H2O i.e. beta-casomorphin Homo sapiens YPVEPF + Ile
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.23 22
-
 room temperature, assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.17.23 22
-
 recombinant enzyme, at room temperature, stable for 6 h Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.23 2
-
 angiotensin I pH 6.5, room temperature Homo sapiens
3.4.17.23 6840
-
 (7-methoxycoumarin-4-yl)acetyl-APK(2,4-dinitrophenyl)-OH pH 6.5, room temperature Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.23 6.5
-
-
 Homo sapiens