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Literature summary extracted from

  • Gazaryan, I.G.; Krasnikov, B.F.; Ashby, G.A.; Thorneley, R.N.; Kristal, B.S.; Brown, A.M.
    Zinc is a potent inhibitor of thiol oxidoreductase activity and stimulates reactive oxygen species production by lipoamide dehydrogenase (2002), J. Biol. Chem., 277, 10064-10072.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.4 Zn2+ stimulates the oxygen oxidase activity of the enzyme Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 NAD+ product inhibition of the oxygen oxidase activity Sus scrofa
1.8.1.4 Zn2+ reversible binding, competitive to lipoamide, uncompetitive to NADH Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 additional information
-
 additional information dehydrogenase and oxidase activity kinetics Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 further purification of the commercial preparation by gel filtration Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.4 protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ ping pong mechanism Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.4 commercial preparation
-
 Sus scrofa
-
1.8.1.4 heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 DL-6,8-thiooctic acid amide + NADH
-
 Sus scrofa ? + NAD+
-
 r
1.8.1.4 lipoamide + NADH
-
 Sus scrofa dihydrolipoamide + NAD+
-
 r
1.8.1.4 O2 + NADH the enzyme produces reactive oxygen species, i.e. hydrogen peroxide, acting as an oxidase Sus scrofa H2O2 + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 LADH
-
 Sus scrofa
1.8.1.4 lipoamide dehydrogenase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.4 20
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.4 7.5
-
 assay at Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD
-
 Sus scrofa
1.8.1.4 NAD+
-
 Sus scrofa
1.8.1.4 NADH
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.4 additional information
-
 additional information Zn2+ inhibition kinetics and mechanism Sus scrofa