EC Number | Cloned (Comment) | Organism |
---|---|---|
7.3.2.1 | overexpression in Escherichia coli | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.3.2.1 | D188K | no ATP hydrolyzing ability | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.3.2.1 | 5'-p-fluorosulfonylbenzoyl adenosine | pH 7.4, 30 min at 30°C, 1-3 mM | Mycobacterium tuberculosis | |
7.3.2.1 | guanidine hydrochloride | 5 min at 100°C, 8000 mM, significant reduction in the activity | Mycobacterium tuberculosis | |
7.3.2.1 | Mn2+ | at room temperature | Mycobacterium tuberculosis | |
7.3.2.1 | additional information | no inhibition with sodium azide, sodium orthovanadate and N-ethylmaleimide | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.3.2.1 | 0.0239 | - |
ATP | at 37 °C, pH 7.5 | Escherichia coli | |
7.3.2.1 | 0.0715 | - |
ATP | at 37 °C, pH 7.5 | Mycobacterium tuberculosis | |
7.3.2.1 | 0.13 | - |
CTP | at 37 °C, pH 7.5 | Mycobacterium tuberculosis | |
7.3.2.1 | 0.211 | - |
GTP | at 37 °C, pH 7.5 | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.3.2.1 | inclusion body | - |
Escherichia coli | 16234 | - |
7.3.2.1 | inclusion body | - |
Mycobacterium tuberculosis | 16234 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.3.2.1 | additional information | no activation with Ca2+, Mg2+ | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
7.3.2.1 | 30500 | - |
PstB is the ATP-binding component, 2 * 30500, SDS-PAGE | Mycobacterium tuberculosis |
7.3.2.1 | 69300 | - |
gel filtration chromatography | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.1 | ATP + H2O + phosphate/out | Escherichia coli | ATP-consuming import of phosphate | ADP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | ATP + H2O + phosphate/out | Mycobacterium tuberculosis | ATP-consuming import of phosphate | ADP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | ATP + H2O + phosphate/out | Mycobacterium tuberculosis H37Rv | ATP-consuming import of phosphate | ADP + phosphate + phosphate/in | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.3.2.1 | Escherichia coli | - |
- |
- |
7.3.2.1 | Mycobacterium tuberculosis | - |
- |
- |
7.3.2.1 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
7.3.2.1 | no modification | - |
Escherichia coli |
7.3.2.1 | no modification | - |
Mycobacterium tuberculosis |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.3.2.1 | by gel filtration chromatography using Superdex 200 column | Escherichia coli |
7.3.2.1 | by gel permeation chromatography using Superdex 200 column | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.1 | ATP + H2O + phosphate/out | - |
Escherichia coli | ADP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | ATP + H2O + phosphate/out | - |
Mycobacterium tuberculosis | ADP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | ATP + H2O + phosphate/out | ATP-consuming import of phosphate | Escherichia coli | ADP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | ATP + H2O + phosphate/out | ATP-consuming import of phosphate | Mycobacterium tuberculosis | ADP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | ATP + H2O + phosphate/out | - |
Mycobacterium tuberculosis H37Rv | ADP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | ATP + H2O + phosphate/out | ATP-consuming import of phosphate | Mycobacterium tuberculosis H37Rv | ADP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | CTP + H2O + phosphate/out | - |
Mycobacterium tuberculosis | CDP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | CTP + H2O + phosphate/out | - |
Mycobacterium tuberculosis H37Rv | CDP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | GTP + H2O + phosphate/out | - |
Mycobacterium tuberculosis | GDP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | GTP + H2O + phosphate/out | - |
Mycobacterium tuberculosis H37Rv | GDP + phosphate + phosphate/in | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.3.2.1 | dimer | PstB is the ATP-binding component, 2 * 30500, SDS-PAGE | Mycobacterium tuberculosis |
7.3.2.1 | More | PstB is the ATP binding component | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.3.2.1 | phosphate-specific transporter | - |
Escherichia coli |
7.3.2.1 | phosphate-specific transporter | - |
Mycobacterium tuberculosis |
7.3.2.1 | Pst | - |
Escherichia coli |
7.3.2.1 | Pst | - |
Mycobacterium tuberculosis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
7.3.2.1 | 24 | 80 | pH 7.5, for 5 min, enzyme is heat-resistant | Mycobacterium tuberculosis |
7.3.2.1 | 37 | - |
pH 7.5, for 5 min, substantial loss in the enzyme activity at temperatures above 37°C | Escherichia coli |
7.3.2.1 | 80 | - |
pH 7.5 | Mycobacterium tuberculosis |
7.3.2.1 | 80 | - |
pH 7.5, between 30 and 60 min of heat, maximum loss of enzyme activity, 60% of initial value | Escherichia coli |
7.3.2.1 | 80 | - |
pH 7.5, incubating for 15 min, proteins are precipitated | Escherichia coli |
7.3.2.1 | 80 | - |
pH 7.5, thermal denaturation is evident within 5 min | Escherichia coli |
7.3.2.1 | 80 | - |
pH 7.5,incubating for 15 min, hardly shows any aggregation and for 1 h, loss 11% of its initial enzyme activity | Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
7.3.2.1 | 5 | 10 | - |
Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.3.2.1 | 0.4 | - |
5'-p-fluorosulfonylbenzoyl adenosine | pH 7.4, at 30°C | Mycobacterium tuberculosis |