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Literature summary extracted from

  • Ito, Y.; Habuchi, O.
    Purification and characterization of N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase from the squid cartilage (2000), J. Biol. Chem., 275, 34728-34736.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.33 Ba2+ 2.5 to 3.5fold activation squid
2.8.2.33 Ca2+ 2.5 to 3.5fold activation squid
2.8.2.33 Co2+ 2.5 to 3.5fold activation squid
2.8.2.33 KCl 2fold activation squid
2.8.2.33 Mg2+ 2.5 to 3.5fold activation squid
2.8.2.33 Mn2+ 2.5 to 3.5fold activation squid
2.8.2.33 NaCl 2fold activation squid
2.8.2.33 protamine 2fold activation squid
2.8.2.33 Sr2+ 2.5 to 3.5fold activation squid

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.33 0.0005
-
 3'-phosphoadenylyl sulfate pH 6.8, 25°C squid
2.8.2.33 0.0011
-
 chondroitin sulfate A pH 6.8, 25°C squid
2.8.2.33 0.0013
-
 dermatan sulfate pH 6.8, 25°C squid

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.33 63000
-
 1 * 63000, SDS-PAGE squid
2.8.2.33 66000
-
 gel filtration squid

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.33 squid
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.8.2.33 glycoprotein contains N-linked oligosaccharides squid

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.33
-
 squid

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.33 cartilage
-
 squid
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.33 3'-phosphoadenylyl sulfate + 4-sulfated chondroitin pentasaccharide best substrate, sulfation only with nonreducing terminal GalNAc(4-SO4) residues squid adenosine 3',5'-bisphosphate + 4,6'-sulfated chondroitin pentasaccharide
-
 ?
2.8.2.33 3'-phosphoadenylyl sulfate + 4-sulfated chondroitin trisaccharide 95.2% of activity compared with 4-sulfated pentasaccharide squid adenosine 3',5'-bisphosphate + 4,6'-sulfated chondroitin trisaccharide
-
 ?
2.8.2.33 3'-phosphoadenylyl sulfate + chondroitin sulfate A
-
 squid adenosine 3',5'-bisphosphate + chondroitin sulfate A 6'-sulfate
-
 ?
2.8.2.33 3'-phosphoadenylyl sulfate + chondroitin sulfate C
-
 squid adenosine 3',5'-bisphosphate + chondroitin sulfate C 6'-sulfate
-
 ?
2.8.2.33 3'-phosphoadenylyl sulfate + dermatan sulfate
-
 squid adenosine 3',5'-bisphosphate + 6'-sulfated dermatan sulfate
-
 ?
2.8.2.33 additional information sulfatation occurs mainly at position 6 of the internal N-acetylgalactosamine 4-sulfate residue squid ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.33 monomer 1 * 63000, SDS-PAGE squid

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.33 6.2
-
-
 squid