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Literature summary extracted from

  • Hamano, Y.; Hoshino, Y.; Nakamori, S.; Takagi, H.
    Overexpression and characterization of an aminoglycoside 6'-N-acetyltransferase with broad specificity from an epsilon-poly-L-lysine producer, Streptomyces albulus IFO14147 (2004), J. Biochem., 136, 517-524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.82 expression in Escherichia coli Streptomyces albulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.00038
-
dibekacin pH 8.0, 30°C Streptomyces albulus
2.3.1.82 0.00052
-
neomycin B pH 8.0, 30°C Streptomyces albulus
2.3.1.82 0.00324
-
sisomicin pH 8.0, 30°C Streptomyces albulus
2.3.1.82 0.00353
-
tobramycin pH 8.0, 30°C Streptomyces albulus
2.3.1.82 0.00434
-
amikacin pH 8.0, 30°C Streptomyces albulus
2.3.1.82 0.0074
-
kanamycin A pH 8.0, 30°C Streptomyces albulus
2.3.1.82 0.0161
-
n-propionyl-CoA pH 8.0, 30°C, cosubstrate: sisomycin Streptomyces albulus
2.3.1.82 0.0187
-
acetyl-CoA pH 8.0, 30°C, cosubstrate: sisomycin Streptomyces albulus
2.3.1.82 0.04188
-
lividomycin A pH 8.0, 30°C Streptomyces albulus
2.3.1.82 0.0686
-
n-butyryl-CoA pH 8.0, 30°C, cosubstrate: sisomycin Streptomyces albulus
2.3.1.82 0.58
-
malonyl-CoA pH 8.0, 30°C, cosubstrate: sisomycin Streptomyces albulus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Streptomyces albulus Q764C8 IFO14147
-
2.3.1.82 Streptomyces albulus IFO14147 Q764C8 IFO14147
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.82 His-tagged AAC(6')-Isa Streptomyces albulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + amikacin
-
Streptomyces albulus CoA + N6'-acetylamikacin
-
?
2.3.1.82 acetyl-CoA + amikacin
-
Streptomyces albulus IFO14147 CoA + N6'-acetylamikacin
-
?
2.3.1.82 acetyl-CoA + dibekacin
-
Streptomyces albulus CoA + N6'-acetyldibekacin
-
?
2.3.1.82 acetyl-CoA + dibekacin
-
Streptomyces albulus IFO14147 CoA + N6'-acetyldibekacin
-
?
2.3.1.82 acetyl-CoA + kanamycin A
-
Streptomyces albulus CoA + N6'-acetylkanamycin A
-
?
2.3.1.82 acetyl-CoA + kanamycin A
-
Streptomyces albulus IFO14147 CoA + N6'-acetylkanamycin A
-
?
2.3.1.82 acetyl-CoA + lividomycin A
-
Streptomyces albulus CoA + O6'-acetyllividomycin A
-
?
2.3.1.82 acetyl-CoA + neomycin B
-
Streptomyces albulus CoA + ?
-
?
2.3.1.82 acetyl-CoA + sisomicin
-
Streptomyces albulus CoA + N6'-acetylsisomicin
-
?
2.3.1.82 acetyl-CoA + tobramycin
-
Streptomyces albulus CoA + N6'-acetyltobramycin
-
?
2.3.1.82 acetyl-CoA + tobramycin
-
Streptomyces albulus IFO14147 CoA + N6'-acetyltobramycin
-
?
2.3.1.82 malonyl-CoA + sisomycin
-
Streptomyces albulus CoA + ?
-
?
2.3.1.82 additional information the enzyme is susceptoble to mild base hydrolysis, suggesting that the enzyme also catalyzes O-acetyltransfer Streptomyces albulus ?
-
?
2.3.1.82 additional information the enzyme is susceptoble to mild base hydrolysis, suggesting that the enzyme also catalyzes O-acetyltransfer Streptomyces albulus IFO14147 ?
-
?
2.3.1.82 n-butyryl-CoA + sisomycin
-
Streptomyces albulus CoA + ?
-
?
2.3.1.82 n-propionyl-CoA + sisomycin
-
Streptomyces albulus CoA + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.82 AAC(6')-Isa
-
Streptomyces albulus
2.3.1.82 aminoglycoside 6'-N-acetyltransferase
-
Streptomyces albulus