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Literature summary extracted from

  • Morii, S.; Sawamoto, S.; Yamauchi, Y.; Miyamoto, M.; Iwami, M.; Itagaki, E.
    Steroid monooxygenase of Rhodococcus rhodochrous: sequencing of the genomic DNA, and hyperexpression, purification, and characterization of the recombinant enzyme (1999), J. Biochem., 126, 624-631.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.54 expressed in Escherichia coli BL21(DE3) Rhodococcus rhodochrous

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.54 0.00044
-
 NADPH pH 8.3, 37°C Rhodococcus rhodochrous
1.14.13.54 0.055
-
 progesterone pH 8.3, 37°C Rhodococcus rhodochrous

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.54 60000
-
 x * 60000, SDS-PAGE Rhodococcus rhodochrous
1.14.13.54 60133
-
 x * 60133, calculated from the deduced amino acid sequence Rhodococcus rhodochrous
1.14.13.54 60919
-
 x * 60919, holoenzyme, calculated from the deduced amino acid sequence Rhodococcus rhodochrous

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.54 progesterone + NADPH + H+ + O2 Rhodococcus rhodochrous
-
 Testosterone acetate + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.54 Rhodococcus rhodochrous
-
 starin IFO 2228
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.54 recombinant enzyme from Escherichia coli Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.54 0.95
-
 purified enzyme Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.54 progesterone + NADPH + H+ + O2
-
 Rhodococcus rhodochrous Testosterone acetate + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.54 ? x * 60000, SDS-PAGE Rhodococcus rhodochrous
1.14.13.54 ? x * 60133, calculated from the deduced amino acid sequence Rhodococcus rhodochrous
1.14.13.54 ? x * 60919, holoenzyme, calculated from the deduced amino acid sequence Rhodococcus rhodochrous

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.54 FAD
-
 Rhodococcus rhodochrous
1.14.13.54 NADPH
-
 Rhodococcus rhodochrous