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Literature summary extracted from

  • Weinberg, M.V.; Jenney, F.E., Jr.; Cui, X.; Adams, M.W.W.
    Rubrerythrin from the hyperthermophilic archaeon Pyrococcus furiosus is a rubredoxin-dependent, iron-containing peroxidase (2004), J. Bacteriol., 186, 7888-7895.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.7 expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.035
-
 H2O2 85°C, pH 7.5 Pyrococcus furiosus
1.11.1.7 0.07
-
 rubredoxin 85°C, pH 7.5 Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.7 Iron 3 iron atoms per subunit, native enzyme Pyrococcus furiosus
1.11.1.7 Zinc 2.5 zinc atoms and 0.5 iron atoms per subunit, recombinant enzyme from cells grown on M-Zn medium Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 19000
-
 2 * 19000, SDS-PAGE Pyrococcus furiosus
1.11.1.7 19377
-
 2 * 19377, mass spectroscopy Pyrococcus furiosus
1.11.1.7 36000
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 native and recombinant enzyme Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.7 36
-
 at 85°C and pH 7.5 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 rubredoxin + H2O2
-
 Pyrococcus furiosus ? + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.7 dimer 2 * 19000, SDS-PAGE Pyrococcus furiosus
1.11.1.7 dimer 2 * 19377, mass spectroscopy Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 rubrerythrin
-
 Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 7.8
-
-
 Pyrococcus furiosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.7 6 10.5 approx. 60% of maximal activity at pH 6.0 and pH 8.0, approx. 30% of maximal activity at pH 9.5, almost no activity at pH 10.5 Pyrococcus furiosus