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Literature summary extracted from

  • Uehara, T.; Park, J.T.
    The N-acetyl-D-glucosamine kinase of Escherichia coli and its role in murein recycling (2004), J. Bacteriol., 186, 7273-7279.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.59 DNA and amino acid sequence determination and analysis, overexpression in strain XL1-Blue Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.59 additional information deletion mutants lack enzyme activity in the cytoplasm, but do not accumulate N-acetyl-D-glucosamine suggesting the existence of an alternative pathway for reutilization of N-acetyl-D-glucosamine, overview Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.59 ZnSO4
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.59 0.34
-
 N-acetyl-D-glucosamine pH 7.8, 37°C Escherichia coli
2.7.1.59 0.9
-
 ATP pH 7.8, 37°C Escherichia coli
2.7.1.59 37.2
-
 D-glucose pH 7.8, 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.59 cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.59 Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.59 ATP + N-acetyl-D-glucosamine Escherichia coli enzyme is important in cell wall murein recycling, 60% of the the murein of the side wall is degraded ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.59 Escherichia coli
-
 gene nagK
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.59 native enzyme 842fold from K12 strain MG1655 in a multistep procedure, recombinant enzyme from strain XL1-Blue Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.59 0.0183
-
 wild-type cell extract Escherichia coli
2.7.1.59 33.3
-
 purified native enzyme Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.59 -80°C, purified recombinant enzyme frozen in dry-ice/ethanol, fully stable for months, thawed samples retain full activity in 40% glycerol at -20°C for a few months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.59 ATP + D-glucose 21% of the activity with N-acetyl-D-glucosamine Escherichia coli ADP + D-glucose 6-phosphate
-
 ?
2.7.1.59 ATP + N-acetyl-D-glucosamine
-
 Escherichia coli ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 ATP + N-acetyl-D-glucosamine enzyme is important in cell wall murein recycling, 60% of the the murein of the side wall is degraded Escherichia coli ADP + N-acetyl-D-glucosamine 6-phosphate
-
 ?
2.7.1.59 additional information poor activity with mannose, galactose, glucosamine, D-mannosamine, N-acetylmannosamine, and N-acetylgalactosamine Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.59 N-acetyl-D-glucosamine kinase
-
 Escherichia coli
2.7.1.59 NagK
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.59 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.59 7.8
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.59 ATP
-
 Escherichia coli