EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.37 | expression in Escherichia coli | Rhodococcus opacus |
1.14.13.167 | expressed in Escherichia coli Rosetta(DE3)pLysS cells | Rhodococcus opacus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.37 | 33200 | - |
x * 33200, deduced from nucleotide sequence | Rhodococcus opacus |
1.14.13.167 | 59700 | - |
x * 59700, calculated from amino acid sequence | Rhodococcus opacus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.37 | Rhodococcus opacus | - |
- |
- |
1.13.11.37 | Rhodococcus opacus SAO101 | - |
- |
- |
1.14.13.167 | Rhodococcus opacus | - |
- |
- |
1.14.13.167 | Rhodococcus opacus SAO101 | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.167 | culture condition:4-nitrophenol-grown cell | - |
Rhodococcus opacus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.167 | 0.0000046 | - |
using 2,4,6-trinitrocatechol as substrate, pH and temperature not specified in the publication | Rhodococcus opacus |
1.14.13.167 | 0.000025 | - |
using 4-nitrocatechol as substrate, pH and temperature not specified in the publication | Rhodococcus opacus |
1.14.13.167 | 0.0000264 | - |
using 4-nitrophenol as substrate, pH and temperature not specified in the publication | Rhodococcus opacus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.37 | benzene-1,2,4-triol + O2 | - |
Rhodococcus opacus | 3-hydroxy-cis,cis-muconate | - |
? | |
1.13.11.37 | benzene-1,2,4-triol + O2 | - |
Rhodococcus opacus SAO101 | 3-hydroxy-cis,cis-muconate | - |
? | |
1.14.13.167 | 2,4,6-trinitrophenol + NADH + H+ + O2 | 17% activity compared to 4-nitrophenol | Rhodococcus opacus | ? | - |
? | |
1.14.13.167 | 2,4,6-trinitrophenol + NADH + H+ + O2 | 17% activity compared to 4-nitrophenol | Rhodococcus opacus SAO101 | ? | - |
? | |
1.14.13.167 | 4-nitrocatechol + NADH + H+ + O2 | 96% activity compared to 4-nitrophenol | Rhodococcus opacus | 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O | - |
? | |
1.14.13.167 | 4-nitrocatechol + NADH + H+ + O2 | 96% activity compared to 4-nitrophenol | Rhodococcus opacus SAO101 | 1,2,4-trihydroxybenzene + nitrite + NAD+ + H2O | - |
? | |
1.14.13.167 | 4-nitrophenol + NADH + H+ + O2 | 100% activity | Rhodococcus opacus | ? | - |
? | |
1.14.13.167 | 4-nitrophenol + NADH + H+ + O2 | 100% activity | Rhodococcus opacus SAO101 | ? | - |
? | |
1.14.13.167 | additional information | almost no activity towards 2,4,6-trinitrophenol | Rhodococcus opacus | ? | - |
? | |
1.14.13.167 | additional information | almost no activity towards 2,4,6-trinitrophenol | Rhodococcus opacus SAO101 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.37 | ? | x * 33200, deduced from nucleotide sequence | Rhodococcus opacus |
1.14.13.167 | ? | x * 59700, calculated from amino acid sequence | Rhodococcus opacus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.37 | NpcC | - |
Rhodococcus opacus |
1.14.13.167 | 4-NP/4-NCA monooxygenase | - |
Rhodococcus opacus |
1.14.13.167 | NpcA | - |
Rhodococcus opacus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.167 | FAD | - |
Rhodococcus opacus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.167 | malfunction | the npcA mutant strain SDA1 completely loses the ability to grow on 4-nitrophenol as the sole carbon source | Rhodococcus opacus |