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Literature summary extracted from

  • Kanai, T.; Ito, S.; Imanaka, T.
    Characterization of a cytosolic NiFe-hydrogenase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2003), J. Bacteriol., 185, 1705-1711.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.1.3
-
Thermococcus kodakarensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.3 cytoplasm exclusively in Thermococcus kodakarensis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.3 Iron NiFe-protein, the alphabetagammadelta subunit contains 0.8 mol of Ni Thermococcus kodakarensis
1.12.1.3 Nickel NiFe-protein, the alphabetagammadelta subunit contains 22.3 mol of Fe Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.1.3 440000
-
gel filtration Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.3 Thermococcus kodakarensis Q8NKS2 alpha-subunit; KOD1
-
1.12.1.3 Thermococcus kodakarensis Q8NKS3 delta-subunit; KOD1
-
1.12.1.3 Thermococcus kodakarensis Q8NKS4 gamma-subunit; KOD1
-
1.12.1.3 Thermococcus kodakarensis Q8NKS5 beta-subunit; KOD1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.3
-
Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.3 H2 + 2,6-dichlorophenolindophenol
-
Thermococcus kodakarensis H+ + ?
-
r
1.12.1.3 H2 + NAD+ specific activity with NAD+ as electron acceptor is 150times lower than that of NADP+ Thermococcus kodakarensis H+ + NADH
-
r
1.12.1.3 H2 + NADP+
-
Thermococcus kodakarensis H+ + NADPH
-
r
1.12.1.3 H2 + oxidized benzyl viologen
-
Thermococcus kodakarensis H+ + reduced benzyl viologen
-
r
1.12.1.3 H2 + oxidized methyl viologen
-
Thermococcus kodakarensis H+ + reduced methyl viologen
-
r
1.12.1.3 H2 + oxidized methylene blue
-
Thermococcus kodakarensis H+ + reduced methylene blue
-
r

Subunits

EC Number Subunits Comment Organism
1.12.1.3 ? (alphabetagammadelta)n, n is 3 or more Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
1.12.1.3 NiFe-hydrogenase
-
Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.1.3 90
-
H2 production with methyl viologen Thermococcus kodakarensis
1.12.1.3 95
-
H2 uptake with methyl viologen Thermococcus kodakarensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.1.3 80
-
203 min, 50% loss of activity of evolution reaction Thermococcus kodakarensis
1.12.1.3 90
-
18 min, 50% loss of activity of evolution reaction Thermococcus kodakarensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.3 FAD the alphabetagammadelta subunit contains 1.01 mol of FAD Thermococcus kodakarensis
1.12.1.3 NAD+ specific activity as electron acceptor is 150times lower than that of NADP+ Thermococcus kodakarensis
1.12.1.3 NADP+ electron acceptor Thermococcus kodakarensis