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Literature summary extracted from

  • Jala, V.R.; Ambili, M.; Prakash, V.; Rao, N.A.; Savithri, H.S.
    Disruption of distal interactions of Arg262 and of substrate binding to Ser52 affect catalysis of sheep liver cytosolic serine hydroxymethyltransferase (2003), Indian J. Biochem. Biophys., 40, 226-237.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 wild-type and mutant enzymes S52A, S52C and R262A are overexpressed in Escherichia coli Ovis aries

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 R262A KM-value for L-Ser is 5.2fold higher than the wild-type value, the turnover-number is 5.25fold lower than the wild-type value, no transamination reaction with D-Ala, reaction with L-allo-Thr is 20fold decreased compared to wild-type value. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme Ovis aries
2.1.2.1 S52A KM-value for L-Ser is 1.3fold higher than the wild-type value, the turnover-number is 6fold lower than the wild-type value, transamination reaction with D-Ala is 20% of the wild-type activity, reaction with L-allo-Thr is 15% of the wild-type activity. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme Ovis aries
2.1.2.1 S52C KM-value for L-Ser is 11.2fold higher than the wild-type value, the turnover-number is 21fold lower than the wild-type value, no transamination reaction with D-Ala, no reaction with L-allo-Thr. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 1
-
L-Ser pH 7.4, 37°C, wild-type enzyme Ovis aries
2.1.2.1 1.3
-
L-Ser pH 7.4, 37°C, mutant enzyme S52A Ovis aries
2.1.2.1 5.2
-
L-Ser pH 7.4, 37°C, mutant enzyme R262A Ovis aries
2.1.2.1 11.2
-
L-Ser pH 7.4, 37°C, mutant enzyme S52C Ovis aries

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytosol
-
Ovis aries 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 53000
-
4 * 53000, SDS-PAGE Ovis aries
2.1.2.1 210000
-
gel filtration Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Ovis aries
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 liver
-
Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,6,7,8-tetrahydrofolate + L-Ser
-
Ovis aries 5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 tetramer 4 * 53000, SDS-PAGE Ovis aries

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 SHMT
-
Ovis aries

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.1 55
-
Tm-value for wild-type enzyme and mutant enzymes R262A, S52C and S52A without ligands or with Gly as ligand. Tm-value for mutant enzymes mutant enzymes R262A, S52C and S52A with L-Ser as ligand Ovis aries
2.1.2.1 68
-
Tm-value for wild-type enzyme with L-Ser as ligand Ovis aries

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 0.2
-
L-Ser pH 7.4, 37°C, mutant enzyme S52C Ovis aries
2.1.2.1 0.7
-
L-Ser pH 7.4, 37°C, mutant enzyme S52A Ovis aries
2.1.2.1 0.8
-
L-Ser pH 7.4, 37°C, mutant enzyme R262A Ovis aries
2.1.2.1 4.2
-
L-Ser pH 7.4, 37°C, wild-type enzyme Ovis aries