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Literature summary extracted from

  • Gutierrez-Correa, J.; Krauth-Siegel, R.L.; Stoppani, A.O.
    Phenothiazine radicals inactivate Trypanosoma cruzi dihydrolipoamide dehydrogenase: enzyme protection by radical scavengers (2003), Free Radic. Res., 37, 281-291.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 expression in Escherichia coli strain JRG 1342 Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 10-(2-dimethylaminopropyl)-dibenzothiazine cation radical 60% inactivation after 10 min incubation and 79% after 30 min using the myeloperoxidase system, 72% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 10-(2-methyl,3-dimethylaminopropyl)-dibenzothiazine cation radical 90% inactivation after 10 min and 30 min incubation using the myeloperoxidase system, 94% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 10-(3-dimethylaminopropyl)-dibenzothiazine cation radical 87% inactivation after 10 min incubation and 89% after 30 min using the myeloperoxidase system, 94% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 2-chloro-10-(3-dimethylaminopropyl)-dibenzothiazine cation radical 45% inactivation after 10 min incubation and 75% after 30 min using the myeloperoxidase system, 89% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 2-chloro-10-[3-(1-methyl-4-piperazinyl)-propyl]-dibenzothiazine cation radical 54% inactivation after 10 min incubation and 80% after 30 min using the myeloperoxidase system, 90% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 2-chloro-10-[3-[1-(2-hydroxyethyl)-4-piperazinyl]propyl]-dibenzothiazine cation radical 42% inactivation after 10 min incubation and 69% after 30 min using the myeloperoxidase system, 79% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 2-methylmercapto-10-[2-(1-methyl-2-piperidinyl)-ethyl]-dibenzothiazine cation radical 77% inactivation after 10 min incubation and 82% after 30 min using the myeloperoxidase system, 85% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 2-propionyl-10-(3-dimethylaminopropyl)-dibenzothiazine cation radical 11% inactivation after 10 min incubation and 32% after 30 min using the myeloperoxidase system, 83% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 2-trifluoromethyl-10-[3-(1-methyl-4-piperazinyl)propyl]-dibenzothiazine cation radical 5% inactivation after 10 min incubation and 16% after 30 min using the myeloperoxidase system, 67% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 2-trifluoromethyl-10-[3-(dimethylamino)propyl]-dibenzothiazine cation radical 2% inactivation after 10 and 30 min incubation using the myeloperoxidase system, 16% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 2-trifluoromethyl-10-[3-[1-(2-hydroxyethyl)4-piperazinyl]propyl]-dibenzothiazine cation radical 1% inactivation after 10 min incubation and 8% after 30 min using the myeloperoxidase system, 61% inactivation after 10 min incubation using the horseradish peroxidase system Trypanosoma cruzi
1.8.1.4 additional information no inhibition by the cation radical of 2-cyano-10-[3-(4-hydroxypiperidinyl)propyl]-dibenzothiazine in either peroxidase system Trypanosoma cruzi
1.8.1.4 phenothiazine cation radicals irreversible inactivation dependent on time, radical structure, and radical production enzyme system, radicals are produced by reaction of myeloperoxidase or horse radish peroxidase on the phenothiazines promazine, trimeprazine, thioridazine, chlorpromazine, prochlorperazine, promethazine, and others, in presence of H2O2, protection by radical scavengers e.g. thiol compounds, amino acids and peptides, pyridine dinucleotides like NADH, or best by ascorbate and trolox, overview Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Trypanosoma cruzi
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.4 400
-
 about, purified recombinant enzyme Trypanosoma cruzi

Storage Stability

EC Number Storage Stability Organism
1.8.1.4 purified recombinant enzyme, -20°C, 100 mM potassium phosphate, 100 mM KCl, pH 7.0, 1 mM EDTA, 50% v/v glycerol, stable for at least 1 year Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 lipoamide + NADH
-
 Trypanosoma cruzi dihydrolipoamide + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 dihydrolipoamide dehydrogenase
-
 Trypanosoma cruzi
1.8.1.4 LADH
-
 Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.4 30
-
 assay at Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.4 7.4
-
 assay at Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 NADH
-
 Trypanosoma cruzi