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Literature summary extracted from

  • Cheng, H.; Jiang, N.; Shen, A.; Feng, Y.
    Molecular cloning and functional expression of D-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli (2005), FEMS Microbiol. Lett., 252, 35-42.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.287 gene ArDH, DNA and amino acid sequence determination and analysis, gene ArDH is part of an operon with several components of a sugar transporter system, subcloning in Escherichia coli strain TG-1, functional expression in Escherichia coli strain BL21(DE3) as His6-tagged enzyme Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.287 12.5
-
 D-arabitol pH 8.5, 25°C Gluconobacter oxydans
1.1.1.287 18.5
-
 D-mannitol pH 8.5, 25°C Gluconobacter oxydans
1.1.1.287 25
-
 D-xylulose pH 6.0, 25°C Gluconobacter oxydans
1.1.1.287 47
-
 D-fructose pH 6.0, 25°C Gluconobacter oxydans
1.1.1.287 125
-
 D-sorbitol pH 8.5, 25°C Gluconobacter oxydans
1.1.1.287 266
-
 meso-erythritol pH 8.5, 25°C Gluconobacter oxydans
1.1.1.287 384
-
 glycerol pH 8.5, 25°C Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.287 membrane bound Gluconobacter oxydans 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.287 29000
-
 x * 29000, recombinant enzyme, SDS-PAGE Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.287 D-arabitinol + NADP+ Gluconobacter oxydans
-
 D-xylulose + NADPH + H+
-
 ?
1.1.1.287 D-arabitinol + NADP+ Gluconobacter oxydans CGMCC 1.110
-
 D-xylulose + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.287 Gluconobacter oxydans Q58LW6
-
-
1.1.1.287 Gluconobacter oxydans CGMCC 1.110 Q58LW6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.287 recombinant His6-tagged enzyme from Escherichia coli Gluconobacter oxydans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.287 culture condition:arabitol-grown cell
-
 Gluconobacter oxydans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.287 2.8
-
 purified recombinant enzyme, substrate glycerol Gluconobacter oxydans
1.1.1.287 3.2
-
 purified recombinant enzyme, substrate meso-erythritol Gluconobacter oxydans
1.1.1.287 6.4
-
 purified recombinant enzyme, substrate D-sorbitol Gluconobacter oxydans
1.1.1.287 7.9
-
 purified recombinant enzyme, substrate D-fructose Gluconobacter oxydans
1.1.1.287 11.4
-
 purified recombinant enzyme, substrate D-xylulose Gluconobacter oxydans
1.1.1.287 23.5
-
 purified recombinant enzyme, substrate D-mannitol Gluconobacter oxydans
1.1.1.287 27.6
-
 purified recombinant enzyme, substrate D-arabitol Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.287 D-arabitinol + NADP+
-
 Gluconobacter oxydans D-xylulose + NADPH + H+
-
 ?
1.1.1.287 D-arabitinol + NADP+
-
 Gluconobacter oxydans CGMCC 1.110 D-xylulose + NADPH + H+
-
 ?
1.1.1.287 D-arabitol + NADP+ preferred substrate for the forward and reverse reaction, respectively Gluconobacter oxydans D-xylulose + NADPH + H+
-
 r
1.1.1.287 D-arabitol + NADP+ preferred substrate for the forward and reverse reaction, respectively Gluconobacter oxydans CGMCC 1.110 D-xylulose + NADPH + H+
-
 r
1.1.1.287 D-mannitol + NADP+ preferred substrate for the forward and reverse reaction, respectively Gluconobacter oxydans D-fructose + NADPH + H+
-
 r
1.1.1.287 D-mannitol + NADP+ preferred substrate for the forward and reverse reaction, respectively Gluconobacter oxydans CGMCC 1.110 D-fructose + NADPH + H+
-
 r
1.1.1.287 D-sorbitol + NADP+
-
 Gluconobacter oxydans sorbose + NADPH + H+
-
 r
1.1.1.287 D-sorbitol + NADP+
-
 Gluconobacter oxydans CGMCC 1.110 sorbose + NADPH + H+
-
 r
1.1.1.287 glycerol + NADP+
-
 Gluconobacter oxydans ? + NADPH + H+
-
 r
1.1.1.287 meso-erythritol + NADP+
-
 Gluconobacter oxydans L-erythrose + NADPH + H+
-
 r
1.1.1.287 additional information substrate specificity for different sugars, overview, no activity with xylitol, L-arabitol, and L-sorbitol Gluconobacter oxydans ?
-
 ?
1.1.1.287 additional information substrate specificity for different sugars, overview, no activity with xylitol, L-arabitol, and L-sorbitol Gluconobacter oxydans CGMCC 1.110 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.287 ? x * 29000, recombinant enzyme, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.287 ArDH
-
 Gluconobacter oxydans
1.1.1.287 D-arabitol dehydrogenase
-
 Gluconobacter oxydans
1.1.1.287 More the enzyme belongs to the short-chain dehydrogenase family Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.287 25
-
 assay at Gluconobacter oxydans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.287 1.28
-
 meso-erythritol pH 8.5, 25°C Gluconobacter oxydans
1.1.1.287 1.33
-
 glycerol pH 8.5, 25°C Gluconobacter oxydans
1.1.1.287 2.57
-
 D-sorbitol pH 8.5, 25°C Gluconobacter oxydans
1.1.1.287 196
-
 D-xylulose pH 6.0, 25°C Gluconobacter oxydans
1.1.1.287 224
-
 D-fructose pH 6.0, 25°C Gluconobacter oxydans
1.1.1.287 243
-
 D-mannitol pH 8.5, 25°C Gluconobacter oxydans
1.1.1.287 258
-
 D-arabitol pH 8.5, 25°C Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.287 6.5
-
 reverse reaction Gluconobacter oxydans
1.1.1.287 8.5
-
 forward reaction Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.287 NADP+ dependent on Gluconobacter oxydans