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Literature summary extracted from
- Cell-free production of active E. coli thioredoxin reductase and glutathione reductase (2004), FEBS Lett., 559, 66-70.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | in-vitro His-tagged enzyme expression | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | additional information | cell-free enzyme synthesis, 71% of which is soluble, the enzyme is dimeric, requires FAD as a cofactor, and is more active than the native enzyme | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | glutathione disulfide + NADPH | Escherichia coli | key enzyme in maintaining the reducing environment in the cell, maintainance of sulfhydryl redox potential in the cytoplasm | glutathione + NADP+ | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.7 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.7 | recombinant His-tagged enzyme from in-vitro expression by nickel chelate affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | additional information | - |
- |
Escherichia coli |
| 1.8.1.7 | 139 | - |
purified in vitro-synthesized enzyme, 30°C, 0.002 mM FAD | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.7 | glutathione disulfide + NADPH | - |
Escherichia coli | glutathione + NADP+ | - |
? | |
| 1.8.1.7 | glutathione disulfide + NADPH | key enzyme in maintaining the reducing environment in the cell, maintainance of sulfhydryl redox potential in the cytoplasm | Escherichia coli | glutathione + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | dimer | homodimer | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.7 | glutathione reductase | - |
Escherichia coli |
| 1.8.1.7 | GR | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 37 | - |
- |
Escherichia coli |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 25 | 37 | increasing activity between 25°C and 37°C | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.7 | 8.1 | - |
assay at | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.7 | FAD | required for activity | Escherichia coli | |
| 1.8.1.7 | NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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