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Literature summary extracted from

  • Jung, S.; Hansel, A.; Kasperczyk, H.; Hoshi, T.; Heinemann, S.H.
    Activity, tissue distribution and site-directed mutagenesis of a human peptide methionine sulfoxide reductase of type B: hCBS1 (2002), FEBS Lett., 527, 91-94.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.12 overexpression of truncated wild-type and mutant enzymes as His-tagged proteins in Escherichia coli, functional coexpression of CBS1 in oocytes with Drosophila melanogaster ShC/B potassium channel Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.12 C105S site-directed mutagenesis, unaltered activity compared to the wild-type enzyme Homo sapiens
1.8.4.12 C169S site-directed mutagenesis, active site mutant, completely inactive mutant Homo sapiens
1.8.4.12 W110A site-directed mutagenesis, reduced activity compared to the wild-type enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.4.12 selenium selenoprotein Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.12 protein L-methionine (R)-sulfoxide + thioredoxin Homo sapiens type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins protein L-methionine + thioredoxin disulfide
-
 ?
1.8.4.12 protein-L-methionine (R)-sulfoxide + dithiothreitol Homo sapiens type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins protein-L-methionine + dithiothreitol disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.12 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.12 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.4.12 L-methionine (R)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O the active site selenocysteine SeC169 is essential for enzyme activity Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.12 heart most abundant in ventricles, interventricular septum, and apex Homo sapiens
-
1.8.4.12 heart ventricle
-
 Homo sapiens
-
1.8.4.12 interventricular septum
-
 Homo sapiens
-
1.8.4.12 kidney
-
 Homo sapiens
-
1.8.4.12 liver
-
 Homo sapiens
-
1.8.4.12 additional information tissue distribution, main expression of CBS1 in muscle tissues, overview Homo sapiens
-
1.8.4.12 skeletal muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.12 protein L-methionine (R)-sulfoxide + thioredoxin type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins Homo sapiens protein L-methionine + thioredoxin disulfide
-
 ?
1.8.4.12 protein-L-methionine (R)-sulfoxide + dithiothreitol type B enzyme CBS1 is stereospecific for the R-stereomer of methionine residues of peptides and proteins Homo sapiens protein-L-methionine + dithiothreitol disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.12 CBS1
-
 Homo sapiens
1.8.4.12 MsrB
-
 Homo sapiens
1.8.4.12 peptide methionine sulfoxide reductase type B
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.4.12 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.4.12 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.12 dithiothreitol
-
 Homo sapiens
1.8.4.12 thioredoxin
-
 Homo sapiens