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Literature summary extracted from

  • Tonon, T.; Sayanova, O.; Michaelson, L.V.; Qing, R.; Harvey, D.; Larson, T.R.; Li, Y.; Napier, J.A.; Graham, I.A.
    Fatty acid desaturases from the microalga Thalassiosira pseudonana (2005), FEBS J., 272, 3401-3412.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.18 expressed in Saccharomyces cerevisiae strain InvSc1 Thalassiosira pseudonana
1.14.19.31 gene TpdesK, encoded on chromosome 5, the gene has a single intron, phylogenetic analysis and tree, recombinant expression in Saccharomyces cerevisiae strain W303A1 Thalassiosira pseudonana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.18 (4E)-sphing-4-enine ceramide + ferrocytochrome b5 + O2 + H+ Thalassiosira pseudonana
-
 (4E,8E)-sphing-4,8-dienine ceramide + ferricytochrome b5 + H2O
-
 ?
1.14.19.18 sphinganine + ferrocytochrome b5 + O2 + H+ Thalassiosira pseudonana the enzyme shows strong preference for dihydroxylated substrates ?
-
 ?
1.14.19.31 docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+ Thalassiosira pseudonana
-
 docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.4 Thalassiosira pseudonana
-
-
-
1.14.19.18 Thalassiosira pseudonana Q4G2T2
-
-
1.14.19.31 Thalassiosira pseudonana
-
 gene TpdesK
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.31 additional information the enzyme is expressed during the exponential phase of growth Thalassiosira pseudonana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.18 (4E)-sphing-4-enine ceramide + ferrocytochrome b5 + O2 + H+
-
 Thalassiosira pseudonana (4E,8E)-sphing-4,8-dienine ceramide + ferricytochrome b5 + H2O
-
 ?
1.14.19.18 sphinganine + ferrocytochrome b5 + O2 + H+ the enzyme shows strong preference for dihydroxylated substrates Thalassiosira pseudonana ?
-
 ?
1.14.19.31 docosa-7,10,13,16,19-pentaenoic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Thalassiosira pseudonana docosa-4,7,10,13,16,19-hexaenoic acid + 2 ferricytochrome b5 + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.4 DELTA8-sphingolipid desaturase
-
 Thalassiosira pseudonana
1.14.19.4 TpdesB
-
 Thalassiosira pseudonana
1.14.19.18 DELTA8-sphingolipid desaturase
-
 Thalassiosira pseudonana
1.14.19.18 DesB
-
 Thalassiosira pseudonana
1.14.19.18 sphinganine DELTA8-desaturase
-
 Thalassiosira pseudonana
1.14.19.31 DELTA4-desaturase
-
 Thalassiosira pseudonana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.18 cytochrome b5
-
 Thalassiosira pseudonana
1.14.19.31 cytochrome b5
-
 Thalassiosira pseudonana

General Information

EC Number General Information Comment Organism
1.14.19.31 evolution the enzyme belongs to the front-end desaturases, phylogenetic analysis and tree, overview. Introns appear to have evolved independently among Tpdes genes Thalassiosira pseudonana
1.14.19.31 metabolism the enzyme is involved in production of the health beneficial polyunsaturated fatty acid docosahexaenoic acid Thalassiosira pseudonana