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Literature summary extracted from

  • Golbik, R.; Meshalkina, L.E.; Sandalova, T.; Tittmann, K.; Fiedler, E.; Neef, H.; Konig, S.; Kluger, R.; Kochetov, G.A.; Schneider, G.; Hubner, G.
    Effect of coenzyme modification on the structural and catalytic properties of wild-type transketolase and of the variant E418A from Saccharomyces cerevisiae (2005), Febs J., 272, 1326-1342.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.2.1.1 wild-type and mutant E418A in complex with N1’-methyl-thiamine diphosphate and wild-type in complex with 4’-methylamino-thiamine diphosphate, topology of active sites remains unchanged by cofactor analogues Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.1 E418A about 0.12% of wild-type activity Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.1 4'-methylamino-thiamine diphosphate cofactor analogue Saccharomyces cerevisiae
2.2.1.1 N1'-methyl-thiamine diphosphate cofactor analogue Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
 Saccharomyces cerevisiae sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 ?
2.2.1.1 hydroxypyruvate + ferricyanide + H2O
-
 Saccharomyces cerevisiae glycolic acid + ferrocyanide + ?
-
 ?
2.2.1.1 hydroxypyruvate + glycolaldehyde
-
 Saccharomyces cerevisiae erythrulose + ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.1 0.002
-
 Hydroxypyruvate mutant E418A, cofactor N1’-methyl-thiamine diphosphate, 25°C, pH 7.6 Saccharomyces cerevisiae
2.2.1.1 0.005
-
 Hydroxypyruvate wild-type, cofactor N1’-methyl-thiamine diphosphate, 25°C, pH 7.6 Saccharomyces cerevisiae
2.2.1.1 0.05
-
 D-xylulose 5-phosphate mutant E418A, cofactor thiamine diphosphate, 25°C, pH 7.6 Saccharomyces cerevisiae
2.2.1.1 0.63
-
 D-xylulose 5-phosphate wild-type, cofactor 4’-methylamino-thiamine diphosphate, 25°C, pH 7.6 Saccharomyces cerevisiae
2.2.1.1 1.8
-
 D-xylulose 5-phosphate mutant E418A, cofactor N1’-methyl-thiamine diphosphate, 25°C, pH 7.6 Saccharomyces cerevisiae
2.2.1.1 2.5
-
 Hydroxypyruvate wild-type, cofactor 4’-methylamino-thiamine diphosphate, 25°C, pH 7.6 Saccharomyces cerevisiae
2.2.1.1 5
-
 D-xylulose 5-phosphate wild-type, cofactor N1’-methyl-thiamine diphosphate, 25°C, pH 7.6 Saccharomyces cerevisiae
2.2.1.1 44.5
-
 Hydroxypyruvate wild-type, cofactor thiamine diphosphate, 25°C, pH 7.6 Saccharomyces cerevisiae
2.2.1.1 45
-
 D-xylulose 5-phosphate wild-type, cofactor thiamine diphosphate, 25°C, pH 7.6 Saccharomyces cerevisiae