EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.59 | 1. binary complex with geranylgeranyl diphosphate, 2. ternary complex with a non-hydrolyzable geranylgeranyl diphosphate analog and CaaX peptide, 3. binary complex with prenyl-peptide product and 4. ternary complex with prenylated product and geranylgeranyl diphosphate. GGTase-I crystals belong to the I222 space group, with three complete 91 kDa heterodimers in the asymmetric unit. GGTase-I crystals are grown at 17°C in hanging drops using equal volumes of protein and reservoir solution (1.3 M (NH4)2SO4, 175 mM Na3 citrate pH 6.5, 20 mM dithiothreitol and 100 mM MES pH 6.3) | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.59 | Zinc | all of the GGTase-I complexes contain a zinc ion bound at full occupancy with a B-factor comparable with the surrounding protein residues. The zinc ion is coordinated by three residues, Asp269b, Cys271b and His321b | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | Rattus norvegicus | GGTase-I catalyzes C-terminal lipidation of more than 100 proteins, including many GTP-binding regulatory proteins | S-geranylgeranyl-protein + diphosphate | - |
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EC Number | Organism | UniProt | Comment | Textmining |
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2.5.1.59 | Rattus norvegicus | - |
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EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | - |
Rattus norvegicus | S-geranylgeranyl-protein + diphosphate | - |
? | |
2.5.1.59 | geranylgeranyl diphosphate + protein-cysteine | GGTase-I catalyzes C-terminal lipidation of more than 100 proteins, including many GTP-binding regulatory proteins | Rattus norvegicus | S-geranylgeranyl-protein + diphosphate | - |
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