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Literature summary extracted from

  • Taylor, J.S.; Reid, T.S.; Terry, K.L.; Casey, P.J.; Beese, L.S.
    Structure of mammalian protein geranylgeranyltransferase type-I (2003), EMBO J., 22, 5963-5974.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.59 1. binary complex with geranylgeranyl diphosphate, 2. ternary complex with a non-hydrolyzable geranylgeranyl diphosphate analog and CaaX peptide, 3. binary complex with prenyl-peptide product and 4. ternary complex with prenylated product and geranylgeranyl diphosphate. GGTase-I crystals belong to the I222 space group, with three complete 91 kDa heterodimers in the asymmetric unit. GGTase-I crystals are grown at 17°C in hanging drops using equal volumes of protein and reservoir solution (1.3 M (NH4)2SO4, 175 mM Na3 citrate pH 6.5, 20 mM dithiothreitol and 100 mM MES pH 6.3) Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.59 Zinc all of the GGTase-I complexes contain a zinc ion bound at full occupancy with a B-factor comparable with the surrounding protein residues. The zinc ion is coordinated by three residues, Asp269b, Cys271b and His321b Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine Rattus norvegicus GGTase-I catalyzes C-terminal lipidation of more than 100 proteins, including many GTP-binding regulatory proteins S-geranylgeranyl-protein + diphosphate
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Organism

EC Number Organism UniProt Comment Textmining
2.5.1.59 Rattus norvegicus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.59 geranylgeranyl diphosphate + protein-cysteine
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Rattus norvegicus S-geranylgeranyl-protein + diphosphate
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2.5.1.59 geranylgeranyl diphosphate + protein-cysteine GGTase-I catalyzes C-terminal lipidation of more than 100 proteins, including many GTP-binding regulatory proteins Rattus norvegicus S-geranylgeranyl-protein + diphosphate
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?