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Literature summary extracted from

  • Chen, H.J.; Chen, Y.M.; Chang, C.M.
    Lipoyl dehydrogenase catalyzes reduction of nitrated DNA and protein adducts using dihydrolipoic acid or ubiquinol as the cofactor (2002), Chem. Biol. Interact., 140, 199-213.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.4 lipoic acid increase in activity might be due to formation of dihydrolipoic acid in the assay system Clostridium kluyveri
1.8.1.4 ubiquinone slight increase in activity might be due to formation of ubiquinol in the assay system Clostridium kluyveri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.4 nitrated DNA + NAD(P)H Sus scrofa enzyme reduces DNA nitro adducts including 8-nitroguanine, 3-nitrotyrosine, and 8-nitroxanthine, which formed in presence of peroxynitrite and nitryl chloride present in inflamed tissues, the nitrated DNA adducts are unstable and undergo spontaneous depurination which can cause cancer, enzyme might be resonsible for reversing biological nitration processes ?
-
 ?
1.8.1.4 nitrated DNA + NADPH Clostridium kluyveri enzyme reduces DNA nitro adducts including 8-nitroguanine, 3-nitrotyrosine, and 8-nitroxanthine, which formed in presence of peroxynitrite and nitryl chloride present in inflamed tissues, the nitrated DNA adducts are unstable and undergo spontaneous depurination DNA + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Clostridium kluyveri
-
-
-
1.8.1.4 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.4 commercial preparation
-
 Sus scrofa
-
1.8.1.4 commercial preparation
-
 Clostridium kluyveri
-
1.8.1.4 heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 3-nitrotyrosine + dihydrolipoic acid
-
 Sus scrofa 3-aminotyrosine + lipoic acid + H2O
-
 ?
1.8.1.4 3-nitrotyrosine + dihydrolipoic acid
-
 Clostridium kluyveri 3-aminotyrosine + lipoic acid + H2O
-
 ?
1.8.1.4 3-nitrotyrosine + NAD(P)H
-
 Sus scrofa 3-aminotyrosine + NAD(P)+ + H2O
-
 ?
1.8.1.4 3-nitrotyrosine + NADPH
-
 Clostridium kluyveri 3-aminotyrosine + NADP+ + H2O
-
 ?
1.8.1.4 3-nitrotyrosine + ubiquinol
-
 Sus scrofa 3-aminotyrosine + ubiquinone + H2O
-
 ?
1.8.1.4 3-nitrotyrosine + ubiquinol
-
 Clostridium kluyveri 3-aminotyrosine + ubiquinone + H2O
-
 ?
1.8.1.4 8-nitroguanine + dihydrolipoic acid
-
 Sus scrofa 8-aminoguanine + lipoic acid + H2O
-
 ?
1.8.1.4 8-nitroguanine + dihydrolipoic acid
-
 Clostridium kluyveri 8-aminoguanine + lipoic acid + H2O
-
 ?
1.8.1.4 8-nitroguanine + NAD(P)H
-
 Sus scrofa 8-aminoguanine + NAD(P)+ + H2O
-
 ?
1.8.1.4 8-nitroguanine + NADPH
-
 Clostridium kluyveri 8-aminoguanine + NADP+ + H2O
-
 ?
1.8.1.4 8-nitroguanine + ubiquinol
-
 Sus scrofa 8-aminoguanine + ubiquinone + H2O
-
 ?
1.8.1.4 8-nitroguanine + ubiquinol
-
 Clostridium kluyveri 8-aminoguanine + ubiquinone + H2O
-
 ?
1.8.1.4 8-nitroxanthine + dihydrolipoic acid
-
 Sus scrofa 8-aminoxanthine + lipoic acid + H2O
-
 ?
1.8.1.4 8-nitroxanthine + dihydrolipoic acid
-
 Clostridium kluyveri 8-aminoxanthine + lipoic acid + H2O
-
 ?
1.8.1.4 8-nitroxanthine + NAD(P)H
-
 Sus scrofa 8-aminoxanthine + NAD(P)+ + H2O
-
 ?
1.8.1.4 8-nitroxanthine + NADPH
-
 Clostridium kluyveri 8-aminoxanthine + NADP+ + H2O
-
 ?
1.8.1.4 8-nitroxanthine + ubiquinol
-
 Sus scrofa 8-aminoxanthine + ubiquinone + H2O
-
 ?
1.8.1.4 8-nitroxanthine + ubiquinol
-
 Clostridium kluyveri 8-aminoxanthine + ubiquinone + H2O
-
 ?
1.8.1.4 additional information substrate specificity, overview Sus scrofa ?
-
 ?
1.8.1.4 additional information substrate specificity, overview Clostridium kluyveri ?
-
 ?
1.8.1.4 nitrated DNA + NAD(P)H enzyme reduces DNA nitro adducts including 8-nitroguanine, 3-nitrotyrosine, and 8-nitroxanthine, which formed in presence of peroxynitrite and nitryl chloride present in inflamed tissues, the nitrated DNA adducts are unstable and undergo spontaneous depurination which can cause cancer, enzyme might be resonsible for reversing biological nitration processes Sus scrofa ?
-
 ?
1.8.1.4 nitrated DNA + NADPH enzyme reduces DNA nitro adducts including 8-nitroguanine, 3-nitrotyrosine, and 8-nitroxanthine, which formed in presence of peroxynitrite and nitryl chloride present in inflamed tissues, the nitrated DNA adducts are unstable and undergo spontaneous depurination Clostridium kluyveri DNA + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 diaphorase
-
 Sus scrofa
1.8.1.4 diaphorase
-
 Clostridium kluyveri
1.8.1.4 lipoamide dehydrogenase
-
 Sus scrofa
1.8.1.4 lipoamide dehydrogenase
-
 Clostridium kluyveri
1.8.1.4 lipoyl dehydrogenase
-
 Sus scrofa
1.8.1.4 lipoyl dehydrogenase
-
 Clostridium kluyveri
1.8.1.4 NAD(P)H:lipoamide oxidoreductase
-
 Sus scrofa
1.8.1.4 NAD(P)H:lipoamide oxidoreductase
-
 Clostridium kluyveri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.4 37
-
 assay at Sus scrofa
1.8.1.4 37
-
 assay at Clostridium kluyveri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.4 7
-
 assay at Sus scrofa
1.8.1.4 7
-
 assay at Clostridium kluyveri

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 dihydrolipoic acid
-
 Sus scrofa
1.8.1.4 dihydrolipoic acid
-
 Clostridium kluyveri
1.8.1.4 additional information no activity with NADH Clostridium kluyveri
1.8.1.4 NAD(P)H NADH is the preferred cofactor Sus scrofa
1.8.1.4 NADPH
-
 Clostridium kluyveri
1.8.1.4 ubiquinol
-
 Sus scrofa
1.8.1.4 ubiquinol
-
 Clostridium kluyveri