EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.33 | gene WecC, amino acid sequence determination and analysis, overexpression of C-terminally His6-tagged enzyme in strain BL21(DE3), phylogenetic analysis | Escherichia coli |
2.6.1.59 | expression in Escherichia coli BL21 | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.33 | (aminooxy)acetic acid | complete inhibition by a mixture of hydroxylamine, (aminooxy)acetic acid, and gabaculine as inhibitors specific for pyridoxal 5'-phosphate-dependent enzymes | Escherichia coli | |
2.6.1.33 | gabaculine | complete inhibition by a mixture of hydroxylamine, (aminooxy)acetic acid, and gabaculine as inhibitors specific for pyridoxal 5'-phosphate-dependent enzymes | Escherichia coli | |
2.6.1.33 | hydroxylamine | complete inhibition by a mixture of hydroxylamine, (aminooxy)acetic acid, and gabaculine as inhibitors specific for pyridoxal 5'-phosphate-dependent enzymes | Escherichia coli | |
2.6.1.59 | (aminooxy)acetic acid | - |
Escherichia coli | |
2.6.1.59 | gabaculine | - |
Escherichia coli | |
2.6.1.59 | hydroxylamine | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.33 | additional information | - |
additional information | kinetics in a coupled assay | Escherichia coli | |
2.6.1.59 | 0.11 | - |
dTDP-4-dehydro-6-deoxy-D-glucose | pH 7.5, 25°C | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.6.1.33 | 44000 | - |
4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry | Escherichia coli |
2.6.1.33 | 44452 | - |
4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry | Escherichia coli |
2.6.1.33 | 44465 | - |
4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry | Escherichia coli |
2.6.1.33 | 180000 | - |
recombinant His6-tagged enzyme, gel filtration | Escherichia coli |
2.6.1.59 | 44000 | - |
4 * 44000, SDS-PAGE | Escherichia coli |
2.6.1.59 | 180000 | - |
gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.33 | Escherichia coli | - |
gene WecC | - |
2.6.1.59 | Escherichia coli | P27833 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.33 | recombinant C-terminally His6-tagged enzyme from strain BL21(DE3) by nickel chelate and hydrophobic interaction chromatography | Escherichia coli |
2.6.1.59 | - |
Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.6.1.33 | dTDP-4-amino-4,6-dideoxy-alpha-D-glucose + 2-oxoglutarate = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + L-glutamate | substrate specificity might be related to conserved motifs VLalpha, VLbeta, and VLgamma, around the active site | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.33 | additional information | - |
activity measurement in a coupled assay with NADH-dependent L-GDH | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.33 | dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate | - |
Escherichia coli | dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate | - |
? | |
2.6.1.33 | dTDP-4-dehydro-6-deoxy-D-mannose + L-glutamate | - |
Escherichia coli | dTDP-4-amino-6-deoxy-D-mannose + 2-oxoglutarate | - |
? | |
2.6.1.33 | additional information | no activity with dGDP-4-keto-6-deoxy-D-mannose, the nucleotide moiety is a key determinant for acceptor substrate specificity | Escherichia coli | ? | - |
? | |
2.6.1.59 | dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate | - |
Escherichia coli | dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate | - |
? | |
2.6.1.59 | dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamine | 65% of the activity with L-glutamate | Escherichia coli | dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutaramate | - |
? | |
2.6.1.59 | dTDP-4-dehydro-6-deoxy-D-mannose + L-glutamate | - |
Escherichia coli | ? + 2-oxoglutarate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.6.1.33 | tetramer | 4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry | Escherichia coli |
2.6.1.59 | tetramer | 4 * 44000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.33 | SAT | - |
Escherichia coli |
2.6.1.33 | sugar aminotransferase | - |
Escherichia coli |
2.6.1.33 | WecE | - |
Escherichia coli |
2.6.1.59 | WecE | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.33 | 37 | - |
- |
Escherichia coli |
2.6.1.59 | 37 | - |
- |
Escherichia coli |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.6.1.33 | 20 | 45 | 25% of maximal activity at 45°C | Escherichia coli |
2.6.1.59 | 20 | 45 | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.59 | 0.38 | - |
dTDP-4-dehydro-6-deoxy-D-glucose | pH 7.5, 25°C | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.33 | 7.5 | - |
- |
Escherichia coli |
2.6.1.59 | 7.5 | - |
- |
Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.6.1.33 | 5 | 9.5 | enzyme shows over 80% of maximal activity at pH 6.0 and pH 8.0, and very low activity below pH 5.0 and above pH 9.5 | Escherichia coli |
2.6.1.59 | 6 | 8 | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.33 | pyridoxal 5'-phosphate | dependent on | Escherichia coli | |
2.6.1.59 | pyridoxal 5'-phosphate | - |
Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.59 | 3.62 | - |
dTDP-4-dehydro-6-deoxy-D-glucose | pH 7.5, 25°C | Escherichia coli |