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Literature summary extracted from

  • Guillaumie, F.; Sterling, J.D.; Jensen, K.J.; Thomas, O.R.T.; Mohnen, D.
    Solid-supported enzymatic synthesis of pectic oligogalacturonides and their analysis by MALDI-TOF mass spectrometry (2003), Carbohydr. Res., 338, 1951-1960.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.43 analysis development of enzyme-bound solid-phase support containing long-pectic oligogalacturonides anchored to the resin material via disulfide-containing cleavable linker, enzyme is reversibly bound, for performance of solid-phase biosynthetic reaction to investigate the pectin oligomer biosynthesis, product identification by MALDI-TOF mass spectrometry, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.43 UDP-D-galacturonate + (1,4-alpha-D-galacturonosyl)n Arabidopsis thaliana involved in biosynthesis of pectin oligomers, overview UDP + (1,4-alpha-D-galacturonosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.43 Arabidopsis thaliana
-
 cv. Columbia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.43 partially from dark-grown cells Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.43 cell suspension culture from cells derived from leaf calli Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.43 0.00095
-
 partially purified enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.43 UDP-D-galacturonate + (1,4-alpha-D-galacturonosyl)n
-
 Arabidopsis thaliana UDP + (1,4-alpha-D-galacturonosyl)n+1
-
 ?
2.4.1.43 UDP-D-galacturonate + (1,4-alpha-D-galacturonosyl)n involved in biosynthesis of pectin oligomers, overview Arabidopsis thaliana UDP + (1,4-alpha-D-galacturonosyl)n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.43 alpha-1,4-galacturonosyltransferase
-
 Arabidopsis thaliana
2.4.1.43 GalAT
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.43 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.43 7.8
-
 assay at Arabidopsis thaliana