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Literature summary extracted from
- Influence of carbon and nitrogen sources on growth, nitrogenase activity, and carbon metabolism of Gluconacetobacter diazotrophicus (2004), Can. J. Microbiol., 50, 745-750.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.6.1 | L-asparagine | 76% inhibition at 10 mM asparagine in the growth medium | Gluconacetobacter diazotrophicus |  |
| 1.18.6.1 | L-aspartic acid | 67% inhibition at 10 mM aspartic acid in the growth medium | Gluconacetobacter diazotrophicus | |
| 1.18.6.1 | L-cysteine | 51% inhibition at 5 mM cysteine in the growth medium | Gluconacetobacter diazotrophicus | |
| 1.18.6.1 | L-Glutamic acid | 68% inhibition at 10 mM glutamic acid in the growth medium | Gluconacetobacter diazotrophicus | |
| 1.18.6.1 | L-glutamine | 60% inhibition at 10 mM glutamine in the growth medium | Gluconacetobacter diazotrophicus | |
| 1.18.6.1 | L-leucine | 54% inhibition at 5 mM leucine in the growth medium | Gluconacetobacter diazotrophicus | |
| 1.18.6.1 | additional information | enzyme activity and bacterial growth is affected by amino acids in the growth medium | Gluconacetobacter diazotrophicus | |
| 1.18.6.1 | NH4+ | 18% inhibition at 5 mM, 32% at 15 mM ammonium in the growth medium | Gluconacetobacter diazotrophicus | |
| 1.18.6.1 | NO3- | 17% inhibition at 15 mM nitrate in the growth medium | Gluconacetobacter diazotrophicus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.18.6.1 | additional information | Gluconacetobacter diazotrophicus | influence of carbon and nitrogen sources on enzyme activity, amino acids in the apoplastic and symplastic sap of sugarcane stems might have a regulatiry role on growth and nitrogenase activity during symbiotic association, overview | ? | - |
? | |
| 1.18.6.1 | additional information | Gluconacetobacter diazotrophicus PAL 5 | influence of carbon and nitrogen sources on enzyme activity, amino acids in the apoplastic and symplastic sap of sugarcane stems might have a regulatiry role on growth and nitrogenase activity during symbiotic association, overview | ? | - |
? | |
| 1.18.6.1 | reduced ferredoxin + H+ + N2 + ATP + H2O | Gluconacetobacter diazotrophicus | biological nitrogen fixation | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir | |
| 1.18.6.1 | reduced ferredoxin + H+ + N2 + ATP + H2O | Gluconacetobacter diazotrophicus PAL 5 | biological nitrogen fixation | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.18.6.1 | Gluconacetobacter diazotrophicus | - |
in endosymbiosis with sugarcane, Saccharum officinarum | - |
| 1.18.6.1 | Gluconacetobacter diazotrophicus PAL 5 | - |
in endosymbiosis with sugarcane, Saccharum officinarum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.18.6.1 | additional information | - |
in vivo activity measurement, quantification of ethylene | Gluconacetobacter diazotrophicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.18.6.1 | acetylene + ? | - |
Gluconacetobacter diazotrophicus | ethylene + ? | - |
? | |
| 1.18.6.1 | acetylene + ? | - |
Gluconacetobacter diazotrophicus PAL 5 | ethylene + ? | - |
? | |
| 1.18.6.1 | additional information | influence of carbon and nitrogen sources on enzyme activity, amino acids in the apoplastic and symplastic sap of sugarcane stems might have a regulatiry role on growth and nitrogenase activity during symbiotic association, overview | Gluconacetobacter diazotrophicus | ? | - |
? | |
| 1.18.6.1 | additional information | influence of carbon and nitrogen sources on enzyme activity, amino acids in the apoplastic and symplastic sap of sugarcane stems might have a regulatiry role on growth and nitrogenase activity during symbiotic association, overview | Gluconacetobacter diazotrophicus PAL 5 | ? | - |
? | |
| 1.18.6.1 | reduced ferredoxin + H+ + N2 + ATP + H2O | - |
Gluconacetobacter diazotrophicus | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir | |
| 1.18.6.1 | reduced ferredoxin + H+ + N2 + ATP + H2O | biological nitrogen fixation | Gluconacetobacter diazotrophicus | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir | |
| 1.18.6.1 | reduced ferredoxin + H+ + N2 + ATP + H2O | - |
Gluconacetobacter diazotrophicus PAL 5 | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir | |
| 1.18.6.1 | reduced ferredoxin + H+ + N2 + ATP + H2O | biological nitrogen fixation | Gluconacetobacter diazotrophicus PAL 5 | oxidized ferredoxin + H2 + NH3 + ADP + phosphate | - |
ir | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.18.6.1 | 30 | - |
in vivo assay at | Gluconacetobacter diazotrophicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.18.6.1 | ATP | - |
Gluconacetobacter diazotrophicus | |
| 1.18.6.1 | Ferredoxin | - |
Gluconacetobacter diazotrophicus |
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