EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.12.18 | additional information | characterization of hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain Comamonas testosteroni LB400 bphA with the homologous gene of Comamonas testosteroni B-356. The C-terminal portion of Burkholderia sp. LB400 alpha-subunit can withstand extensive structural modifications, and that these modifications can change the catalytic properties of the enzyme. Exchanging the C-terminal portion of Comamonas testosteroni B-356 BPDO alpha-subunit with that of Burkholderia sp. LB400 alpha-subunit generates inactive chimeras | Comamonas testosteroni |
1.14.12.18 | additional information | characterization of hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain Comamonas testosteroni LB400 bphA with the homologous gene of Comamonas testosteroni B-356. The C-terminal portion of Burkholderia sp. LB400 alpha-subunit can withstand extensive structural modifications, and that these modifications can change the catalytic properties of the enzyme. Exchanging the C-terminal portion of Comamonas testosteroni B-356 BPDO alpha-subunit with that of Burkholderia sp. LB400 alpha-subunit generates inactive chimeras | Burkholderia sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.18 | Burkholderia sp. | - |
strain LB400, hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain LB400 bphA with the homologous gene of Comamonas testosteroni B-356 | - |
1.14.12.18 | Comamonas testosteroni | - |
strain B-356, hybrid biphenyl dioxygenases obtained by recombining Burkholderia sp. strain LB400 bphA with the homologous gene of Comamonas testosteroni B-356 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.18 | 2,2',5,5'-tetrachlorobiphenyl + NADH + H+ + O2 | - |
Burkholderia sp. | ? | - |
? | |
1.14.12.18 | 2,2'-dichlorobiphenyl + NADH + H+ + O2 | - |
Burkholderia sp. | ? | - |
? | |
1.14.12.18 | 2,2'-dichlorobiphenyl + NADH + H+ + O2 | wild-type enzyme | Comamonas testosteroni | ? | - |
? | |
1.14.12.18 | 3,3'-dichlorobiphenyl + NADH + H+ + O2 | wild-type enzyme | Comamonas testosteroni | ? | - |
? | |
1.14.12.18 | 4,4'-dichlorobiphenyl + NADH + H+ + O2 | - |
Burkholderia sp. | ? | - |
? | |
1.14.12.18 | biphenyl + NADH + H+ + O2 | - |
Burkholderia sp. | ? | - |
? | |
1.14.12.18 | biphenyl + NADH + H+ + O2 | wild-type enzyme | Comamonas testosteroni | ? | - |
? | |
1.14.12.18 | additional information | no activity with 3,3'-dichlorobiphenyl | Burkholderia sp. | ? | - |
? | |
1.14.12.18 | additional information | no activity with 4,4'-dichlorobiphenyl and 2,2',5,5'-tetrachlorobiphenyl | Comamonas testosteroni | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.12.18 | BPDO | - |
Comamonas testosteroni |
1.14.12.18 | BPDO | - |
Burkholderia sp. |