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Literature summary extracted from

  • Ladisch, M.R.; Kohlmann, K.L.
    Recombinant human insulin (1992), Biotechnol. Prog., 8, 469-478.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.17.2 synthesis production of human insulin Sus scrofa
3.4.21.4 synthesis production of human insulin-threonine-ester, which can be simply converted into human insulin by hydrolysis with subsequent purification steps Sus scrofa
3.4.21.4 synthesis production of mono/di-arg insulin Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.2 Sus scrofa
-
-
-
3.4.21.4 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.2 pancreas
-
 Sus scrofa
-
3.4.21.4 pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.2 mono/di-Arg-insulin + H2O
-
 Sus scrofa human insulin
-
 ?
3.4.21.4 pre-Pro-Arg-insulin + H2O
-
 Sus scrofa mono/di-Arg insulin + ?
-
 ?
3.4.21.4 Pro-Arg-insulin + H2O
-
 Sus scrofa mono/di-Arg insulin + ?
-
 ?
3.4.21.4 pro-insulin + H2O
-
 Sus scrofa human insulin-threonine-ester
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.2 30 35
-
 Sus scrofa
3.4.21.4 6
-
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.4 7
-
-
 Sus scrofa
3.4.21.4 8.3
-
-
 Sus scrofa