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Literature summary extracted from

  • Gu, L.; Lajoie, C.; Kelly, C.
    Expression of a Phanerochaete chrysosporium manganese peroxidase gene in the yeast Pichia pastoris (2003), Biotechnol. Prog., 19, 1403-1409.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.13 three differentexpression vectors are constructed: pZBMNP contains the native Phanerochaete chrysosporium fungal secretion signal, palphaAMNP contains an alpha-factor secretion signal derived from Saccharomyces cerevisiae, and pZBIMNP has no secretion signal and is used for intracellular expression Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.13 0.055
-
 H2O2 recombinant enzyme Phanerodontia chrysosporium
1.11.1.13 0.058
-
 H2O2 wild-type enzyme Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.13 46000
-
 x * 46000, SDS-PAGE Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Phanerodontia chrysosporium
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.13 glycoprotein the recombinant enzyme produced by ZBMNP16 is hyperglycosylated Phanerodontia chrysosporium

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.13
-
 Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.13 additional information
-
-
 Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 2,6-dimethoxyphenol + H2O2
-
 Phanerodontia chrysosporium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.13 ? x * 46000, SDS-PAGE Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.13 55
-
 pH 4.5, 3 min, wild-type enzyme loses about 75% of the initial activity, 50% loss of activity after 3 min in presence of 1 mM ferric chloride. pH 4.5, 2 min, recombinant enzyme loses about 90% of the initial activity, about 45% loss of activity in presence of 1 mM ferric chloride Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.13 168
-
 H2O2 recombinant enzyme Phanerodontia chrysosporium
1.11.1.13 237
-
 H2O2 wild-type enzyme Phanerodontia chrysosporium