EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.85 | expression in Escherichia coli, His6-tagged At-SPS2 fusion protein and the truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.85 | 0.000565 | - |
geranylgeranyl diphosphate | pH 8.0, 30°C, His6-tagged At-SPS2 fusion protein | Arabidopsis thaliana | |
2.5.1.85 | 0.000843 | - |
geranylgeranyl diphosphate | pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus), truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | |
2.5.1.85 | 0.00512 | - |
farnesyl diphosphate | pH 8.0, 30°C, His6-tagged At-SPS2 fusion protein | Arabidopsis thaliana | |
2.5.1.85 | 0.00689 | - |
(2E,6E)-farnesyl diphosphate | pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | |
2.5.1.85 | 0.0289 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | |
2.5.1.85 | 0.0377 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, His6-tagged At-SPS2 fusion protein | Arabidopsis thaliana | |
2.5.1.85 | 0.182 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | |
2.5.1.85 | 0.248 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, His6-tagged At-SPS2 fusion protein | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.5.1.85 | chloroplast | At-SPS2 is transported into chloroplasts | Arabidopsis thaliana | 9507 | - |
2.5.1.85 | endoplasmic reticulum | At-SPS1 | Arabidopsis thaliana | 5783 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.85 | 46044 | - |
x * 46044, calculated from sequence | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.85 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | Arabidopsis thaliana | At-SPS1 contributes to the biosynthesis of the ubiquinone side-chain in Arabidopsis thaliana | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.85 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | Arabidopsis thaliana | At-SPS2 supplies the precursor of the plastoquinone side-chain in Arabidopsis thaliana | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.85 | Arabidopsis thaliana | Q76FS5 | - |
- |
2.5.1.85 | Arabidopsis thaliana | Q8S948 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.85 | recombinant His6-tagged At-SPS2 fusion protein partially purified to about 10% purity, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) is purified to about 90% purity | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.85 | leaf | mRNA level is 11fold higher in leaf than in root | Arabidopsis thaliana | - |
2.5.1.85 | leaf | mRNA level is 5fold higher in leaf than in root | Arabidopsis thaliana | - |
2.5.1.85 | root | mRNA level is 11fold higher in leaf than in root | Arabidopsis thaliana | - |
2.5.1.85 | root | mRNA level is 5fold higher in leaf than in root | Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.85 | (2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate | At-SPS2 prefers geranylgeranyl diphosphate to farnesyl diphosphate as the allylic substrate | Arabidopsis thaliana | 6 diphosphate + all-trans-nonaprenyl diphosphate | the main product is nonaprenyl diphosphate, considerable amounts of intermediates, such as geranylgeranyl diphosphate are formed | ? | |
2.5.1.85 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | At-SPS1 contributes to the biosynthesis of the ubiquinone side-chain in Arabidopsis thaliana | Arabidopsis thaliana | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.85 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | At-SPS2 supplies the precursor of the plastoquinone side-chain in Arabidopsis thaliana | Arabidopsis thaliana | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.85 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | At-SPS2 prefers geranylgeranyl diphosphate to farnesyl diphosphate as the allylic substrate | Arabidopsis thaliana | 5 diphosphate + all-trans-nonaprenyl diphosphate | a large amount of nonaprenyl diphosphate is formed | ? | |
2.5.1.85 | additional information | no activity with dimethylallyl diphosphate and geranyl diphosphate, recombinant His6-tagged At-SPS2 fusion protein and truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.85 | ? | x * 46044, calculated from sequence | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.85 | At-SPS1 | - |
Arabidopsis thaliana |
2.5.1.85 | At-SPS2 | - |
Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.85 | 1.72 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | |
2.5.1.85 | 2.33 | - |
geranylgeranyl diphosphate | pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus), truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | |
2.5.1.85 | 2.83 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | |
2.5.1.85 | 3.77 | - |
(2E,6E)-farnesyl diphosphate | pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.85 | 15.5 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | |
2.5.1.85 | 547 | - |
(2E,6E)-farnesyl diphosphate | pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | |
2.5.1.85 | 595 | - |
isopentenyl diphosphate | pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana | |
2.5.1.85 | 2770 | - |
geranylgeranyl diphosphate | pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus), truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) | Arabidopsis thaliana |